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Sperm miR-15a and miR-29b are associated with bull fertility.
- Source :
-
Andrologia [Andrologia] 2020 Feb; Vol. 52 (1), pp. e13412. Date of Electronic Publication: 2019 Oct 31. - Publication Year :
- 2020
-
Abstract
- MicroRNAs modulate male fertility by regulating gene expression. In this study, dynamics of sperm miR-15a, miR-29b and miR-34a from high fertility (HF) and low fertility (LF) bulls using RT-qPCR were evaluated. Bioinformatic tools were employed to ascertain genes of interest of the sperm miRNAs. The expression levels of p53, BCL2, BAX and DNMT1 in bull spermatozoa were determined by immunoblotting. MicroRNA levels of miR-15a and miR-29 were higher in LF sires when compared with those present in HF bulls. Expression levels of miR-34a did not differ between the two groups. We found an inverse correlation between miR-15a and bull fertility. MiR29-b was also negatively associated with fertility scores. BCL2 and DNMT1 were higher in HF bulls while BAX was higher in the LF group. Our data showed a positive correlation between BCL2 and bull fertility. In addition, DNMT1 was positively associated with bull fertility. Furthermore, levels of BAX were negatively linked with bull fertility scores. Identification of miRNAs found in the spermatozoa of sires with different in vivo fertility helps understand the alterations in the fertilising capacity from cattle and other mammals. These potential biomarkers can be used in reproductive biotechnology as fertility markers to assess semen quality and predict male fertility.<br /> (© 2019 Blackwell Verlag GmbH.)
- Subjects :
- Animals
Biomarkers metabolism
Breeding
Computational Biology
DNA (Cytosine-5-)-Methyltransferase 1 genetics
Gene Expression Regulation physiology
Male
Proto-Oncogene Proteins c-bcl-2 genetics
Semen Analysis methods
bcl-2-Associated X Protein genetics
bcl-2-Associated X Protein metabolism
Cattle physiology
Fertility genetics
MicroRNAs metabolism
Semen Analysis veterinary
Spermatozoa metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1439-0272
- Volume :
- 52
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Andrologia
- Publication Type :
- Academic Journal
- Accession number :
- 31671225
- Full Text :
- https://doi.org/10.1111/and.13412