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Sustained release of silibinin-loaded chitosan nanoparticle induced apoptosis in glioma cells.
- Source :
-
Journal of biomedical materials research. Part A [J Biomed Mater Res A] 2020 Mar; Vol. 108 (3), pp. 458-469. Date of Electronic Publication: 2019 Nov 11. - Publication Year :
- 2020
-
Abstract
- In this study, a chitosan nanoparticle formulation was synthesized for loading silibinin as a sustained-release drug system to evaluate its effects on apoptosis in C6 glioma cells. This synthesized nanoparticle was analyzed by measurement methods including Fourier transform infrared (FTIR), field emission-scanning electron microscopy (FE-SEM), dynamic light scattering (DLS), X-ray diffraction (XRD), and differential scanning calorimetry (DSC). The formation and amorphization of nanoparticle were confirmed by FTIR and XRD analysis, respectively. The mean diameter of silibinin-loaded chitosan nanoparticles (SCNP) was 50 ±â€‰7 and 188.6 ±â€‰0.17 nm by using FE-SEM and DLS, respectively. In addition, the positive zeta potential of nanoparticles was +11.5. Rhodamine-conjugated SCNP analysis showed the internalization of silibinin to C6 glioma cells. The cytotoxicity assay indicated that the nanoformulation of silibinin was toxic to C6 glioma cells. Although SCNP significantly increased the expression of the both apoptotic genes in C6 cells, Bax and caspase3, it did not have any significant effect on the level of the antiapoptotic gene, Bcl2. In contrast, SCNP did not have any toxic effect on H9C2 cells. In conclusion, the results of the current study indicated that SCNP can be considered as a sustained-release drug system for future cell-based therapeutic strategies.<br /> (© 2019 Wiley Periodicals, Inc.)
- Subjects :
- Animals
Antineoplastic Agents, Phytogenic pharmacology
Cell Line, Tumor
Humans
Nanoparticles chemistry
Rats
Silybin pharmacology
Antineoplastic Agents, Phytogenic administration & dosage
Apoptosis drug effects
Chitosan chemistry
Delayed-Action Preparations chemistry
Glioma drug therapy
Silybin administration & dosage
Subjects
Details
- Language :
- English
- ISSN :
- 1552-4965
- Volume :
- 108
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Journal of biomedical materials research. Part A
- Publication Type :
- Academic Journal
- Accession number :
- 31657514
- Full Text :
- https://doi.org/10.1002/jbm.a.36827