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Autologous micrograft accelerates endogenous wound healing response through ERK-induced cell migration.
- Source :
-
Cell death and differentiation [Cell Death Differ] 2020 May; Vol. 27 (5), pp. 1520-1538. Date of Electronic Publication: 2019 Oct 25. - Publication Year :
- 2020
-
Abstract
- Defective cell migration causes delayed wound healing (WH) and chronic skin lesions. Autologous micrograft (AMG) therapies have recently emerged as a new effective and affordable treatment able to improve wound healing capacity. However, the precise molecular mechanism through which AMG exhibits its beneficial effects remains unrevealed. Herein we show that AMG improves skin re-epithelialization by accelerating the migration of fibroblasts and keratinocytes. More specifically, AMG-treated wounds showed improvement of indispensable events associated with successful wound healing such as granulation tissue formation, organized collagen content, and newly formed blood vessels. We demonstrate that AMG is enriched with a pool of WH-associated growth factors that may provide the starting signal for a faster endogenous wound healing response. This work links the increased cell migration rate to the activation of the extracellular signal-regulated kinase (ERK) signaling pathway, which is followed by an increase in matrix metalloproteinase expression and their extracellular enzymatic activity. Overall we reveal the AMG-mediated wound healing transcriptional signature and shed light on the AMG molecular mechanism supporting its potential to trigger a highly improved wound healing process. In this way, we present a framework for future improvements in AMG therapy for skin tissue regeneration applications.
- Subjects :
- Animals
Cells, Cultured
Female
Fibroblasts metabolism
Fibroblasts pathology
Gene Expression Profiling
Gene Regulatory Networks
Keratinocytes cytology
Keratinocytes enzymology
MAP Kinase Signaling System genetics
Matrix Metalloproteinases metabolism
Mice, Inbred C57BL
Solubility
Transcription, Genetic
Transplantation, Autologous
Cell Movement genetics
Extracellular Signal-Regulated MAP Kinases metabolism
Skin Transplantation
Wound Healing genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1476-5403
- Volume :
- 27
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Cell death and differentiation
- Publication Type :
- Academic Journal
- Accession number :
- 31654035
- Full Text :
- https://doi.org/10.1038/s41418-019-0433-3