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Minichromosome Maintenance Complex (MCM) Genes Profiling and MCM2 Protein Expression in Cervical Cancer Development.
- Source :
-
Asian Pacific journal of cancer prevention : APJCP [Asian Pac J Cancer Prev] 2019 Oct 01; Vol. 20 (10), pp. 3043-3049. Date of Electronic Publication: 2019 Oct 01. - Publication Year :
- 2019
-
Abstract
- Objective: Minichromosome maintenance complex (MCM) proteins are essential for the process of DNA replication and cell division. This study aimed to evaluate MCM genes expression profiles and MCM2 protein in HPV-associated cervical carcinogenesis.<br />Methodology: MCM2, 4, 5 and 7 genes expression profiles were evaluated in three cervical tissue samples each of normal cervix, human papillomavirus (HPV)-infected low grade squamous intraepithelial lesion (LSIL), high grade squamous intraepithelial lesion (HSIL) and squamous cell carcinoma (SCC), using Human Transcriptome Array 2.0 and validated by nCounter® PanCancer Pathway NanoString Array. Immunohistochemical expression of MCM2 protein was semi-quantitatively assessed by histoscore in tissue microarrays containing 9 cases of normal cervix, 10 LSIL, 10 HSIL and 42 cases of SCC.<br />Results: MCM2, 4, 5 and 7 genes expressions were upregulated with increasing fold change during the progression from LSIL to HSIL and the highest in SCC. MCM2 gene had the highest fold change in SCC compared to normal cervix. Immunohistochemically, MCM2 protein was localised in the nuclei of basal cells of normal cervical epithelium and dysplastic-neoplastic cells of CIN and SCC. There was a significant difference in MCM2 protein expression between the histological groups (P = 0.039), and histoscore was the highest in HSIL compared to normal cervix (P = 0.010).<br />Conclusion: The upregulation of MCM genes expressions in cervical carcinogenesis reaffirms MCM as a proliferative marker in DNA replication pathway, whereby proliferation of dysplastic and cancer cells become increasingly dysregulated and uncontrolled. A strong expression of MCM2 protein in HSIL may aid as a concatenated screening tool in detecting pre-cancerous cervical lesions.
- Subjects :
- Carcinoma, Squamous Cell genetics
Carcinoma, Squamous Cell metabolism
Carcinoma, Squamous Cell virology
Case-Control Studies
Cell Cycle Proteins genetics
Cell Cycle Proteins metabolism
Female
Follow-Up Studies
Humans
Minichromosome Maintenance Complex Component 2 genetics
Minichromosome Maintenance Complex Component 4 genetics
Minichromosome Maintenance Complex Component 4 metabolism
Minichromosome Maintenance Complex Component 7 genetics
Minichromosome Maintenance Complex Component 7 metabolism
Papillomaviridae isolation & purification
Papillomavirus Infections virology
Prognosis
Uterine Cervical Neoplasms genetics
Uterine Cervical Neoplasms metabolism
Uterine Cervical Neoplasms virology
Vaginal Smears
Uterine Cervical Dysplasia genetics
Uterine Cervical Dysplasia metabolism
Uterine Cervical Dysplasia virology
Carcinoma, Squamous Cell pathology
Minichromosome Maintenance Complex Component 2 metabolism
Papillomavirus Infections complications
Uterine Cervical Neoplasms pathology
Uterine Cervical Dysplasia pathology
Subjects
Details
- Language :
- English
- ISSN :
- 2476-762X
- Volume :
- 20
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- Asian Pacific journal of cancer prevention : APJCP
- Publication Type :
- Academic Journal
- Accession number :
- 31653153
- Full Text :
- https://doi.org/10.31557/APJCP.2019.20.10.3043