Azospirillum thermophilum sp. nov., isolated from a hot spring.

A novel Gram-staining negative, aerobic, motile by flagellum, rod-shaped bacterium, designated CFH 70021 ^T was isolated from a hot spring soil sample collected from Tengchong, Yunnan province, PR China. Growth of CFH 70021 ^T occurred at 15-50 °C (optimum 50 °C), pH 5.0-7.0 (optimum pH 7.0) and with 0-3.0 % (w/v) NaCl (optimum 0 %, w/v). The genome of CFH 70021 ^T consisted of four complete circular chromosomes and five plasmids, the genomic DNA G+C content was 69.3 mol%. Comparison of the 16S rRNA gene sequences indicated that CFH 70021 ^T represented a member of the genus Azospirillum and showed close relationship with the type strains of Azospirillum agricola CC-HIH038 ^T (97.8 %), Azospirillum rugosum IMMIB AFH-6 ^T (97.6 %), Azospirillum doebereinerae GSF71 ^T (97.6 %), Azospirillum thiophilum DSM 21654 ^T (97.4 %) and Azospirillum picis IMMIB TAR-3 ^T (97.2 %). The polar lipids of CFH 70021 ^T contained diphosphatidylglycerol, phosphatidylmehtylethanolamine, phosphatidylglycerol, phosphatidylcholine, two aminolipids and an unidentified phospholipid. The predominant cellular fatty acids (>10 %) included C _19:0 cyclo ω 8c (11.4 %), C _16 : 0 (27.6 %) and summed feature 8 (C _18:1 ω7 c /C _18:1 ω6 c , 40.9 %). The major isoprenoid quinone was Q-10. On the basis of the low ANIb result (<78 %) and different phenotypic and chemotaxonomic characters, we conclude that strain CFH 70021 ^T represents a novel member of the genus Azospirillum , for which the name Azospirillum thermophilum sp. nov. is proposed. The type strain is CFH 70021 ^T (=KCTC 62259 ^T = CCTCC AB2018121 ^T ).