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Quantitation of TLR4 Internalization in Response to LPS in Thioglycollate Elicited Peritoneal mouse Macrophages by Flow Cytometry.
- Source :
-
Bio-protocol [Bio Protoc] 2019 Sep 20; Vol. 9 (18). - Publication Year :
- 2019
-
Abstract
- Internalization of cell surface Toll Like Receptor 4 (TLR4) is a convenient read-out to measure LPS dependent activation of the TRIF adaptor pathway. We here provide a protocol to quantify the LPS dependent internalization of TLR4 using thioglycollate-elicited peritoneal macrophages by flow cytometry.
Details
- Language :
- English
- ISSN :
- 2331-8325
- Volume :
- 9
- Issue :
- 18
- Database :
- MEDLINE
- Journal :
- Bio-protocol
- Publication Type :
- Academic Journal
- Accession number :
- 31649967
- Full Text :
- https://doi.org/10.21769/BioProtoc.3369