Back to Search
Start Over
Comprehensive immune profiling and immune-monitoring using body fluid of patients with metastatic gastric cancer.
- Source :
-
Journal for immunotherapy of cancer [J Immunother Cancer] 2019 Oct 21; Vol. 7 (1), pp. 268. Date of Electronic Publication: 2019 Oct 21. - Publication Year :
- 2019
-
Abstract
- Background: The aim of this study is to profile the cytokines and immune cells of body fluid from metastatic gastric cancer (mGC), and evaluate the potential role as a prognostic factor and the feasibility as a predictive biomarker or monitoring source for immune checkpoint inhibitor.<br />Methods: Body fluid including ascites and pleural fluid were obtained from 55 mGC patients and 24 matched blood. VEGF-A, IL-10, and TGF-β1 were measured and immune cells were profiled by fluorescence assisted cell sorting (FACS).<br />Results: VEGF-A and IL-10 were significantly higher in body fluid than in plasma of mGC. Proportion of T lymphocytes with CD69 or PD-1, memory T cell marked with CD45RO, and number of Foxp3+ T regulatory cells (Tregs) were significantly higher in body fluid than those in blood of mGC. Proportion of CD8 T lymphocyte with memory marker (CD45RO) and activation marker (HLA-DR), CD3 T lymphocyte with PD-1, and number of FoxP3+ Tregs were identified as independent prognostic factors. When patients were classified by molecular subgroups of primary tumor, VEGF-A was significantly higher in genomically stable (GS)-like group than that in chromosomal instability (CIN)-like group while PD-L1 positive tumor cells (%) showed opposite results. Monitoring immune dynamics using body fluid was also feasible. Early activated T cell marked with CD25 was significantly increased in chemotherapy treated group.<br />Conclusions: By analyzing cytokines and proportion of immune cells in body fluid, prognosis of patients with mGC can be predicted. Immune monitoring using body fluid may provide more effective treatment for patients with mGC.
- Subjects :
- Adult
Aged
Antineoplastic Agents, Immunological therapeutic use
Ascitic Fluid cytology
CD4-Positive T-Lymphocytes immunology
Case-Control Studies
Cytokines immunology
Cytokines isolation & purification
Drug Resistance, Neoplasm immunology
Feasibility Studies
Female
Healthy Volunteers
Humans
Kaplan-Meier Estimate
Lymphocyte Activation
Lymphocyte Count
Male
Middle Aged
Pleural Effusion, Malignant blood
Prognosis
Stomach Neoplasms drug therapy
Stomach Neoplasms mortality
Stomach Neoplasms pathology
T-Lymphocytes, Regulatory immunology
Antineoplastic Agents, Immunological pharmacology
Ascitic Fluid immunology
Monitoring, Immunologic methods
Pleural Effusion, Malignant immunology
Stomach Neoplasms immunology
Subjects
Details
- Language :
- English
- ISSN :
- 2051-1426
- Volume :
- 7
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Journal for immunotherapy of cancer
- Publication Type :
- Academic Journal
- Accession number :
- 31639056
- Full Text :
- https://doi.org/10.1186/s40425-019-0708-8