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The long non-coding RNA GHSROS facilitates breast cancer cell migration and orthotopic xenograft tumour growth.
- Source :
-
International journal of oncology [Int J Oncol] 2019 Dec; Vol. 55 (6), pp. 1223-1236. Date of Electronic Publication: 2019 Oct 04. - Publication Year :
- 2019
-
Abstract
- Recent evidence suggests that numerous long non‑coding RNAs (lncRNAs) are dysregulated in cancer, and have critical roles in tumour development and progression. The present study investigated the ghrelin receptor antisense lncRNA growth hormone secretagogue receptor opposite strand (GHSROS) in breast cancer. Reverse transcription‑quantitative polymerase chain reaction revealed that GHSROS expression was significantly upregulated in breast tumour tissues compared with normal breast tissue. Induced overexpression of GHSROS in the MDA‑MB‑231 breast cancer cell line significantly increased cell migration in vitro, without affecting cell proliferation, a finding similar to our previous study on lung cancer cell lines. Microarray analysis revealed a significant repression of a small cluster of major histocompatibility class II genes and enrichment of immune response pathways; this phenomenon may allow tumour cells to better evade the immune system. Ectopic overexpression of GHSROS in the MDA‑MB‑231 cell line significantly increased orthotopic xenograft growth in mice, suggesting that in vitro culture does not fully capture the function of this lncRNA. This study demonstrated that GHSROS may serve a relevant role in breast cancer. Further studies are warranted to explore the function and therapeutic potential of this lncRNA in breast cancer progression.
- Subjects :
- Animals
Apoptosis genetics
Breast pathology
Breast Neoplasms immunology
Breast Neoplasms pathology
Disease Progression
Down-Regulation
Female
Gene Expression Profiling
Histocompatibility Antigens Class II genetics
Histocompatibility Antigens Class II immunology
Humans
MCF-7 Cells
Mice
Middle Aged
Oligonucleotide Array Sequence Analysis
Receptors, Ghrelin genetics
Tumor Escape genetics
Xenograft Model Antitumor Assays
Breast Neoplasms genetics
Cell Movement genetics
Gene Expression Regulation, Neoplastic
RNA, Long Noncoding metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1791-2423
- Volume :
- 55
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- International journal of oncology
- Publication Type :
- Academic Journal
- Accession number :
- 31638176
- Full Text :
- https://doi.org/10.3892/ijo.2019.4891