A flow cytometric granularity assay for the quantification of infectious virus.

Authors :: Logan M
Manalil J
Notte C
Kearse C
George S
Zeiser A
Farrell P
Aucoin MG
Source :: Vaccine [Vaccine] 2019 Nov 08; Vol. 37 (47), pp. 7090-7099. Date of Electronic Publication: 2019 Apr 16.
Publication Year :: 2019
Abstract: A flow cytometry-based assay was developed to assess the infective titer of two recombinant viruses: a recombinant herpes simplex type 2 (rHSV-2) and a recombinant canary pox (rALVAC.gfp). This method uses granularity of infected Vero and QT-35 cells, respectively, and correlates this to the infectious titer of virus samples. The percent of the cell populations with a high level of granularity could accurately be correlated to viral titers obtained through a traditional plaque assay, with R <superscript>2</superscript> values greater than 0.8 using a semi-logarithmic scale. This approach offers a rapid, high-throughput method for infectious virus titration with similar accuracy to a traditional plaque assay.<br /> (Copyright © 2019 The Authors. Published by Elsevier Ltd.. All rights reserved.)