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Development of a water-soluble near-infrared fluorescent probe for endogenous cysteine imaging.
- Source :
-
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy [Spectrochim Acta A Mol Biomol Spectrosc] 2020 Feb 05; Vol. 226, pp. 117544. Date of Electronic Publication: 2019 Oct 10. - Publication Year :
- 2020
-
Abstract
- We designed and synthesized a water-soluble near-infrared (NIR) fluorescent probe with the recognition unit of the cyanine-like structure and acrylate group. Through an aromatic ring nucleophilic substitution reaction based on sulfhydryl moiety, an off-on fluorescence response toward cysteine (Cys) was realized. The probe exhibited excellent spectral performance with an emission wavelength of 720nm and a detection limit of 0.20μM. The spectral properties, selectivity and anti-interference performance of the probe were systematically investigated. Density functional theory (DFT) calculations were conducted to clarify the luminescence mechanism of the probe. Furthermore, the probe was successfully applied to the detection of free Cys in human serum and the NIR imaging of endogenous Cys in living cells. Thus, the probe has a promising application prospect in clinical diagnosis and fluorescence imaging.<br /> (Copyright © 2019 Elsevier B.V. All rights reserved.)
- Subjects :
- Cell Line, Tumor
Cysteine metabolism
Fluorescence
Humans
Infrared Rays
Limit of Detection
Single-Cell Analysis methods
Solubility
Spectroscopy, Near-Infrared methods
Water chemistry
Cysteine analysis
Fluorescent Dyes isolation & purification
Fluorescent Dyes pharmacology
Molecular Imaging methods
Subjects
Details
- Language :
- English
- ISSN :
- 1873-3557
- Volume :
- 226
- Database :
- MEDLINE
- Journal :
- Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy
- Publication Type :
- Academic Journal
- Accession number :
- 31629982
- Full Text :
- https://doi.org/10.1016/j.saa.2019.117544