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Blocking LFA-1 Aggravates Cardiac Inflammation in Experimental Autoimmune Myocarditis.
- Source :
-
Cells [Cells] 2019 Oct 17; Vol. 8 (10). Date of Electronic Publication: 2019 Oct 17. - Publication Year :
- 2019
-
Abstract
- The lymphocyte function-associated antigen 1 (LFA-1) is a member of the beta2-integrin family and plays a pivotal role for T cell activation and leukocyte trafficking under inflammatory conditions. Blocking LFA-1 has reduced or aggravated inflammation depending on the inflammation model. To investigate the effect of LFA-1 in myocarditis, mice with experimental autoimmune myocarditis (EAM) were treated with a function blocking anti-LFA-1 antibody from day 1 of disease until day 21, the peak of inflammation. Cardiac inflammation was evaluated by measuring infiltration of leukocytes into the inflamed cardiac tissue using histology and flow cytometry and was assessed by analysis of the heart weight/body weight ratio. LFA-1 antibody treatment severely enhanced leukocyte infiltration, in particular infiltration of CD11b+ monocytes, F4/80+ macrophages, CD4+ T cells, Ly6G+ neutrophils, and CD133+ progenitor cells at peak of inflammation which was accompanied by an increased heart weight/body weight ratio. Thus, blocking LFA-1 starting at the time of immunization severely aggravated acute cardiac inflammation in the EAM model.
- Subjects :
- AC133 Antigen metabolism
Animals
Body Weight drug effects
CD11b Antigen metabolism
CD4-Positive T-Lymphocytes drug effects
CD4-Positive T-Lymphocytes metabolism
Flow Cytometry
Inflammation immunology
Inflammation pathology
Leukemic Infiltration immunology
Leukemic Infiltration pathology
Macrophages drug effects
Macrophages metabolism
Male
Mice
Mice, Inbred BALB C
Monocytes drug effects
Monocytes metabolism
Neutrophils drug effects
Neutrophils metabolism
Organ Size drug effects
Stem Cells drug effects
Stem Cells metabolism
Anti-Bacterial Agents pharmacology
Autoimmune Diseases immunology
Autoimmune Diseases pathology
Lymphocyte Function-Associated Antigen-1 metabolism
Nervous System Autoimmune Disease, Experimental immunology
Nervous System Autoimmune Disease, Experimental pathology
Subjects
Details
- Language :
- English
- ISSN :
- 2073-4409
- Volume :
- 8
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- Cells
- Publication Type :
- Academic Journal
- Accession number :
- 31627327
- Full Text :
- https://doi.org/10.3390/cells8101267