Generation of three human induced pluripotent stem cell sublines (MZT04D, MZT04J, MZT04C) for reproductive science research.

Authors :: Pandolfi EC
Rojas EJ
Sosa E
Gell JJ
Hunt TJ
Goldsmith S
Fan Y
Silber SJ
Clark AT
Source :: Stem cell research [Stem Cell Res] 2019 Oct; Vol. 40, pp. 101576. Date of Electronic Publication: 2019 Sep 16.
Publication Year :: 2019
Abstract: We generated three human induced pluripotent stem cell (hiPSC) sublines from human dermal fibroblasts (HDFs) (MZT04) generated from a skin biopsy donated from a previously fertile woman. The skin biopsy was broadly consented for generating hiPSC lines for biomedical research, including unique consent specifically for studying human fertility, infertility and germ cells. hiPSCs were reprogrammed using Sendai virus vectors and were subsequently positive for markers of self-renewal including OCT4, NANOG, TRA-1-81 and SSEA-4. Pluripotency was further verified using teratomas and PluriTest. These sublines serve as controls for hiPSC research projects aimed at understanding the cell and molecular regulation of female fertility and infertility.<br /> (Copyright © 2019 The Authors. Published by Elsevier B.V. All rights reserved.)

Subjects :: Cell Differentiation
Cell Line metabolism
Cells, Cultured
Cellular Reprogramming
Female
Fibroblasts cytology
Fibroblasts metabolism
Homozygote
Humans
Induced Pluripotent Stem Cells metabolism
Male
Middle Aged
Octamer Transcription Factor-3 genetics
Octamer Transcription Factor-3 metabolism
SOXB1 Transcription Factors genetics
SOXB1 Transcription Factors metabolism
Cell Line cytology
Induced Pluripotent Stem Cells cytology

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