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Depletion of the FtsH1/3 Proteolytic Complex Suppresses the Nutrient Stress Response in the Cyanobacterium Synechocystis sp strain PCC 6803.
- Source :
-
The Plant cell [Plant Cell] 2019 Dec; Vol. 31 (12), pp. 2912-2928. Date of Electronic Publication: 2019 Oct 15. - Publication Year :
- 2019
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Abstract
- The membrane-embedded FtsH proteases found in bacteria, chloroplasts, and mitochondria are involved in diverse cellular processes including protein quality control and regulation. The genome of the model cyanobacterium Synechocystis sp PCC 6803 encodes four FtsH homologs designated FtsH1 to FtsH4. The FtsH3 homolog is present in two hetero-oligomeric complexes: FtsH2/3, which is responsible for photosystem II quality control, and the essential FtsH1/3 complex, which helps maintain Fe homeostasis by regulating the level of the transcription factor Fur. To gain a more comprehensive insight into the physiological roles of FtsH hetero-complexes, we performed genome-wide expression profiling and global proteomic analyses of Synechocystis mutants conditionally depleted of FtsH3 or FtsH1 grown under various nutrient conditions. We show that the lack of FtsH1/3 leads to a drastic reduction in the transcriptional response to nutrient stress of not only Fur but also the Pho, NdhR, and NtcA regulons. In addition, this effect is accompanied by the accumulation of the respective transcription factors. Thus, the FtsH1/3 complex is of critical importance for acclimation to iron, phosphate, carbon, and nitrogen starvation in Synechocystis. plantcell;31/12/2912/FX1F1fx1.<br /> (© 2019 American Society of Plant Biologists. All rights reserved.)
- Subjects :
- Acclimatization genetics
Bacterial Proteins genetics
Carbon deficiency
Carbon metabolism
Gene Expression
Metalloproteases genetics
Mutation
Nitrogen deficiency
Nitrogen metabolism
Nutrients metabolism
Phosphate-Binding Proteins genetics
Phosphate-Binding Proteins metabolism
Phosphates deficiency
Phosphates metabolism
Phosphorylation
Photosystem II Protein Complex chemistry
Photosystem II Protein Complex genetics
Proteolysis
Proteome genetics
Proteome metabolism
Proteomics
Regulon genetics
Repressor Proteins genetics
Ribosomal Proteins genetics
Ribosomal Proteins metabolism
Synechocystis enzymology
Transcription Factors genetics
Transcription Factors metabolism
Bacterial Proteins metabolism
Gene Expression Regulation, Bacterial genetics
Metalloproteases metabolism
Nutrients deficiency
Photosystem II Protein Complex metabolism
Repressor Proteins metabolism
Synechocystis metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1532-298X
- Volume :
- 31
- Issue :
- 12
- Database :
- MEDLINE
- Journal :
- The Plant cell
- Publication Type :
- Academic Journal
- Accession number :
- 31615847
- Full Text :
- https://doi.org/10.1105/tpc.19.00411