mEosEM withstands osmium staining and Epon embedding for super-resolution CLEM.

Authors :: Fu Z
Peng D
Zhang M
Xue F
Zhang R
He W
Xu T
Xu P
Source :: Nature methods [Nat Methods] 2020 Jan; Vol. 17 (1), pp. 55-58. Date of Electronic Publication: 2019 Oct 14.
Publication Year :: 2020
Abstract: Super-resolution correlative light and electron microscopy (SR-CLEM) is a powerful approach for imaging specific molecules at the nanoscale in the context of the cellular ultrastructure. Epon epoxy resin embedding offers advantages for SR-CLEM, including ultrastructural preservation and high quality sectioning. However, Epon embedding eliminates fluorescence from most fluorescent proteins. We describe a photocontrollable fluorescent protein, mEosEM, that can survive Epon embedding after osmium tetroxide (OsO <subscript>4</subscript> ) treatment for improved SR-CLEM.