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Extracellular matrix turnover and inflammation in chemically-induced TMJ arthritis mouse models.
- Source :
-
PloS one [PLoS One] 2019 Oct 11; Vol. 14 (10), pp. e0223244. Date of Electronic Publication: 2019 Oct 11 (Print Publication: 2019). - Publication Year :
- 2019
-
Abstract
- The temporomandibular joint (TMJ) is a fibrocartilaginous tissue critical for chewing and speaking. In patients with temporomandibular disorders (TMDs), permanent tissue loss can occur. Recapitulating the complexity of TMDs in animal models is difficult, yet critical for the advent of new therapies. Synovial fluid from diseased human samples revealed elevated levels of tumor necrosis factor alpha (TNF-alpha). Here, we propose to recapitulate these findings in mice by subjecting murine TMJs with TNF-alpha or CFA (Complete Freund's Adjuvant) in mandibular condyle explant cultures and by local delivery in vivo using TMJ intra-articular injections. Both TNF-alpha and CFA delivery to whole mandibular explants and in vivo increased extracellular matrix deposition and increased cartilage thickness, while TNF-alpha treated explants had increased expression of inflammatory cytokines and degradative enzymes. Moreover, the application of TNF-alpha or CFA in both models reduced cell number. CFA delivery in vivo caused soft tissue inflammation, including pannus formation. Our work provides two methods of chemically induced TMJ inflammatory arthritis through a condyle explant model and intra-articular injection model that replicate findings seen in synovial fluid of human patients, which can be used for further studies delineating the mechanisms underlying TMJ pathology.<br />Competing Interests: The authors have declared that no competing interests exists.
- Subjects :
- ADAMTS5 Protein genetics
ADAMTS5 Protein immunology
Adolescent
Adult
Aged
Animals
Arthritis, Experimental chemically induced
Arthritis, Experimental genetics
Cartilage, Articular drug effects
Cartilage, Articular pathology
Collagen Type II genetics
Collagen Type II immunology
Collagen Type X genetics
Collagen Type X immunology
Disease Models, Animal
Extracellular Matrix drug effects
Extracellular Matrix pathology
Female
Freund's Adjuvant administration & dosage
Gene Expression drug effects
Gene Expression immunology
Humans
Interleukins genetics
Interleukins immunology
Male
Mandibular Condyle drug effects
Mandibular Condyle immunology
Mandibular Condyle pathology
Mice
Mice, Inbred C57BL
Middle Aged
Synovial Fluid immunology
Temporomandibular Joint drug effects
Temporomandibular Joint pathology
Temporomandibular Joint Disorders genetics
Temporomandibular Joint Disorders pathology
Tissue Culture Techniques
Tumor Necrosis Factor-alpha administration & dosage
Arthritis, Experimental immunology
Cartilage, Articular immunology
Extracellular Matrix immunology
Temporomandibular Joint immunology
Temporomandibular Joint Disorders immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1932-6203
- Volume :
- 14
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- PloS one
- Publication Type :
- Academic Journal
- Accession number :
- 31603905
- Full Text :
- https://doi.org/10.1371/journal.pone.0223244