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Blue laser light inhibits biofilm formation in vitro and in vivo by inducing oxidative stress.
- Source :
-
NPJ biofilms and microbiomes [NPJ Biofilms Microbiomes] 2019 Oct 09; Vol. 5 (1), pp. 29. Date of Electronic Publication: 2019 Oct 09 (Print Publication: 2019). - Publication Year :
- 2019
-
Abstract
- Resolution of bacterial infections is often hampered by both resistance to conventional antibiotic therapy and hiding of bacterial cells inside biofilms, warranting the development of innovative therapeutic strategies. Here, we report the efficacy of blue laser light in eradicating Pseudomonas aeruginosa cells, grown in planktonic state, agar plates and mature biofilms, both in vitro and in vivo, with minimal toxicity to mammalian cells and tissues. Results obtained using knock-out mutants point to oxidative stress as a relevant mechanism by which blue laser light exerts its anti-microbial effect. Finally, the therapeutic potential is confirmed in a mouse model of skin wound infection. Collectively, these data set blue laser phototherapy as an innovative approach to inhibit bacterial growth and biofilm formation, and thus as a realistic treatment option for superinfected wounds.<br />Competing Interests: Competing interestsG.O. has part-time employment, V.M. has full-time employment in K-Laser d.o.o. (Sežana, Slovenia). The remaining authors declare no competing interests.<br /> (© The Author(s) 2019.)
- Subjects :
- Animals
Cell Line
Culture Media
Disease Models, Animal
Humans
Mice, Inbred C57BL
Models, Biological
Pseudomonas Infections therapy
Radiotherapy methods
Treatment Outcome
Wound Infection therapy
Biofilms growth & development
Biofilms radiation effects
Lasers
Light
Oxidative Stress
Pseudomonas aeruginosa growth & development
Pseudomonas aeruginosa radiation effects
Subjects
Details
- Language :
- English
- ISSN :
- 2055-5008
- Volume :
- 5
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- NPJ biofilms and microbiomes
- Publication Type :
- Academic Journal
- Accession number :
- 31602310
- Full Text :
- https://doi.org/10.1038/s41522-019-0102-9