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Upregulation of LncRNA FAM83H-AS1 in hepatocellular carcinoma promotes cell proliferation, migration and invasion by Wnt/β-catenin pathway.
- Source :
-
European review for medical and pharmacological sciences [Eur Rev Med Pharmacol Sci] 2019 Sep; Vol. 23 (18), pp. 7855-7862. - Publication Year :
- 2019
-
Abstract
- Objective: The long non-coding RNA, FAM83H antisense RNA 1 (head to head) (FAM83H-AS1), has been reported to function as an oncogene in some types of cancer. However, the role of lncRNA FAM83H-AS1 in hepatocellular carcinoma (HCC) still remains unknown. The present work aims to explore the effect of lncRNA FAM83H-AS1 on cell proliferation and cell invasion in HCC.<br />Patients and Methods: 66 pairs of HCC tissue samples and adjacent normal tissues were collected, and the expression level of lncRNA FAM83H-AS1 was detected by quantitative Reverse Transcription-Polymerase Chain Reaction (qRT-PCR) analysis. Cell Counting Kit-8 (CCK-8) assay was performed to detect cell proliferation ability, and transwell assays were applied to observe the effect of lncRNA FAM83H-AS1 on cell migration and invasion. QRT-PCR and Western blot analysis was used to determine the mRNA and protein expression.<br />Results: In the present study, our results confirmed that lncRNA FAM83H-AS1 expression was overexpressed in HCC tissues relative to the adjacent normal tissues. Furthermore, higher lncRNA FAM83H-AS1 expression significantly associated with tumor size and vascular invasion in patients with HCC. The Kaplan-Meier methods and log rank test demonstrated that increased lncRNA FAM83H-AS1 expression associated with shorter patient overall survival compared to lower lncRNA FAM83H-AS1 expression in patients with HCC. Moreover, function assays by CCK-8 cell proliferation and transwell cell migration and invasion assays showed that the knockdown of lncRNA FAM83H-AS1 significantly inhibited cell proliferation, migration, and invasion ability in HCC. Moreover, we found that the downregulating expression of lncRNA FAM83H-AS1 inhibited Wnt/β-catenin pathway by reducing β-catenin and WNT1 expression in HCC cells.<br />Conclusions: Together, our results indicated that it plays an important role in HCC progression and may be a potential target for HCC treatment.
- Subjects :
- Adult
Aged
Carcinoma, Hepatocellular mortality
Case-Control Studies
Cell Movement genetics
Cell Proliferation genetics
China epidemiology
Down-Regulation
Female
Gene Expression Regulation, Neoplastic genetics
Humans
Male
Middle Aged
Neoplasm Invasiveness genetics
Neoplasm Staging methods
RNA, Antisense genetics
Up-Regulation
Wnt Signaling Pathway genetics
beta Catenin metabolism
Carcinoma, Hepatocellular genetics
Liver Neoplasms pathology
Proteins genetics
RNA, Long Noncoding genetics
Subjects
Details
- Language :
- English
- ISSN :
- 2284-0729
- Volume :
- 23
- Issue :
- 18
- Database :
- MEDLINE
- Journal :
- European review for medical and pharmacological sciences
- Publication Type :
- Academic Journal
- Accession number :
- 31599410
- Full Text :
- https://doi.org/10.26355/eurrev_201909_18995