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Line Immunoassay for Confirmation and Discrimination of Human T-Cell Lymphotropic Virus Infections in Inconclusive Western Blot Serum Samples from Brazil.
- Source :
-
Journal of clinical microbiology [J Clin Microbiol] 2019 Dec 23; Vol. 58 (1). Date of Electronic Publication: 2019 Dec 23 (Print Publication: 2019). - Publication Year :
- 2019
-
Abstract
- Difficulties in confirming and discriminating human T-cell lymphotropic virus type 1 (HTLV-1) and HTLV-2 infections by serological Western blot (WB) assays (HTLV Blot 2.4; MP Biomedicals) have been reported in Brazil, mainly in HIV/AIDS patients, with a large number of WB-indeterminate and WB-positive but HTLV-untypeable results. Nonetheless, a line immunoassay (LIA) (INNO-LIA HTLV-I/II; Fujirebio) provided enhanced specificity and sensitivity for confirming HTLV-1/2 infections. To add information concerning the improved ability of the LIA in relation to WB when applied to samples of individuals from different risk groups from Brazil, we performed the present study. Three groups were analyzed: group 1 (G1), with 62 samples from HIV/AIDS patients from São Paulo, SP (48 WB indeterminate and 14 HTLV untypeable); group 2 (G2), with 24 samples from patients with hepatitis B or hepatitis C from São Paulo (21 WB indeterminate and 3 HTLV untypeable; 17 HIV seropositive); and group 3 (G3), with 25 samples from an HTLV outpatient clinic in Salvador, Bahia (16 WB indeterminate and 9 HTLV untypeable; all HIV seronegative). Overall, the LIA confirmed HTLV-1/2 infection (HTLV-1, HTLV-2, or HTLV) in 66.1% (G1), 83.3% (G2), and 76.0% (G3) of samples. Interestingly, the majority of WB-indeterminate results were confirmed by the LIA as being HTLV-2 positive in G1 and G2 but not in G3, in which the samples were defined as being HTLV-1 or HTLV positive. These results agree with the virus types that circulate in such patients of different regions in Brazil and emphasize that the LIA is the best serological test for confirming HTLV-1 and HTLV-2 infections, independently of being applied in HTLV-monoinfected or HTLV-coinfected individuals.<br /> (Copyright © 2019 Campos et al.)
- Subjects :
- Adolescent
Adult
Aged
Aged, 80 and over
Brazil epidemiology
Coinfection epidemiology
Female
Humans
Male
Mass Screening
Middle Aged
Sensitivity and Specificity
Serologic Tests
Young Adult
HTLV-I Infections epidemiology
HTLV-I Infections virology
HTLV-II Infections epidemiology
HTLV-II Infections virology
Human T-lymphotropic virus 1 immunology
Human T-lymphotropic virus 2 immunology
Immunoassay methods
Subjects
Details
- Language :
- English
- ISSN :
- 1098-660X
- Volume :
- 58
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Journal of clinical microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 31597749
- Full Text :
- https://doi.org/10.1128/JCM.01384-19