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Molecular Characterization of Fasciola and Dicrocoelium Species Isolated from Ruminant Livestock in Qazvin, Iran.
- Source :
-
Infectious disorders drug targets [Infect Disord Drug Targets] 2020; Vol. 20 (5), pp. 737-742. - Publication Year :
- 2020
-
Abstract
- Introduction: Fascioliasis and dicrocoeliasis are the most frequent zoonotic diseases with increasing human health problems in different parts of Iran. Two species, Fasciola hepatica (F. hepatica) and Fasciola gigantica (F. gigantica), are spread in the country. Molecular approaches have a decisive role in identifying both the species. The aim of this study was to detect Fasciola spp. and Dicrocoelium spp. by amplifying the ITS-2 and 28S rDNA gene sequence.<br />Methods: Overall, 30 infected liver samples were collected from the livestock of Qazvin, Iran. The adult flukes were collected from different livestock. DNA extraction and PCR amplification of ribosomal RNA gene region (ITS2) and 28S rDNA gene fragment were conducted and a phylogenetic tree was constructed.<br />Result: All the isolates obtained from the cattle (No: 7) and 82.6% (No: 19) of sheep isolates were infected with F. hepatica species, whereas 17.4% (No: 4) of sheep isolates were infected with F. gigantica. It was also shown that F. hepatica was the predominant species of Fasciola present in the region. All the specimens were infected with Dicrocoelium dendriticum (D. dendriticum).<br />Conclusion: Both the species of Fasciola were found in Qazvin. D. dendriticum was the sole infecting species of the Dicrocoelium genus in the livestock of the city of Qazvin. Further research studies are needed to determine the intermediate host of the parasites in the region.<br /> (Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.net.)
- Subjects :
- Animals
Cattle
DNA, Protozoan genetics
DNA, Ribosomal genetics
Dicrocoelium genetics
Dicrocoelium isolation & purification
Fasciola genetics
Fasciola isolation & purification
Humans
Iran
Liver parasitology
Multilocus Sequence Typing
Phylogeny
Sheep
Zoonoses parasitology
DNA, Ribosomal Spacer genetics
Dicrocoeliasis parasitology
Dicrocoelium classification
Fasciola classification
Fascioliasis parasitology
Livestock parasitology
RNA, Ribosomal, 28S genetics
Subjects
Details
- Language :
- English
- ISSN :
- 2212-3989
- Volume :
- 20
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Infectious disorders drug targets
- Publication Type :
- Academic Journal
- Accession number :
- 31595853
- Full Text :
- https://doi.org/10.2174/1871526519666191009143043