Streptomyces bauhiniae sp. nov., isolated from tree bark of Bauhinia variegata Linn. in Thailand.

A novel actinomycete strain, Bv016 ^T , belonging to the genus Streptomyces , was isolated from the bark of tree, Bauhinia variegata Linn., collected in Thailand. The taxonomic position of the strain was characterized by using a polyphasic approach. ll-Diaminopimelic acid, glucose, mannose, ribose and galactose were detected in its whole-cell hydrolysates. The N -acyl type of muramic acid was acetyl. The strain contained anteiso-C _{15  :  0} , iso-C _{16  :  0} , iso-C _{15  :  0} and iso-C _{14  :  0} as the major fatty acids and MK-9(H ₈ ), MK-9(H ₆ ) and MK-9(H ₄ ) as the major menaquinones. The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannoside. The strain was closely related to Streptomyces griseoluteus JCM 4041 ^T (98.4 %), Streptomyces seoulensis JCM 10116 ^T (98.4 %) and Streptomyces recifensis JCM 4408 ^T (98.2 %). The draft genome of Bv016 ^T was 6.74 Mb with 5949 coding sequences with an average G+C content of 71.7 mol%. The ANIb and ANIm values of strain Bv016 ^T were 94.1 and 95.2 %, respectively, and the digital DNA-DNA hybridization value was 60.6 % in comparison with the draft genome of S. griseoluteus JCM 4765 ^T . The results of the taxonomic analysis suggested that strain Bv016 ^T represented a novel species of the genus Streptomyces for which the name Streptomyces bauhiniae sp. nov. is proposed. The type strain is Bv016 ^T (=JCM 33208 ^T =TISTR 2645 ^T ).