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Single-Cell Transcriptome Analysis of Uniparental Embryos Reveals Parent-of-Origin Effects on Human Preimplantation Development.

Authors :
Leng L
Sun J
Huang J
Gong F
Yang L
Zhang S
Yuan X
Fang F
Xu X
Luo Y
Bolund L
Peters BA
Lu G
Jiang T
Xu F
Lin G
Source :
Cell stem cell [Cell Stem Cell] 2019 Nov 07; Vol. 25 (5), pp. 697-712.e6. Date of Electronic Publication: 2019 Oct 03.
Publication Year :
2019

Abstract

To investigate the contribution of parental genomes to early embryogenesis, we profiled the single-cell transcriptomes of human biparental and uniparental embryos systematically from the 1-cell to the morula stage. We observed that uniparental embryos exhibited variable and decreased embryonic genome activation (EGA). Comparative transcriptome analysis identified 807 maternally biased expressed genes (MBGs) and 581 paternally biased expressed genes (PBGs) in the preimplantation stages. MBGs became apparent at the 4-cell stage and contributed to the initiation of EGA, whereas PBGs preferentially appeared at the 8-cell stage and might affect embryo compaction and trophectoderm specification. Regulatory network analysis revealed that DUX4, EGR2, and DUXA are key transcription factors in MBGs' expression; ZNF263 and KLF3 are important for PBGs' expression. We demonstrated that parent-specific DNA methylation might account for the expression of most PBGs. Our results provide a valuable resource to understand parental genome activation and might help to elucidate parent-of-origin effects in early human development.<br /> (Copyright © 2019 Elsevier Inc. All rights reserved.)

Details

Language :
English
ISSN :
1875-9777
Volume :
25
Issue :
5
Database :
MEDLINE
Journal :
Cell stem cell
Publication Type :
Academic Journal
Accession number :
31588047
Full Text :
https://doi.org/10.1016/j.stem.2019.09.004