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Multiparametric radiobiological assays show that variation of X-ray energy strongly impacts relative biological effectiveness: comparison between 220 kV and 4 MV.

Authors :
Paget V
Ben Kacem M
Dos Santos M
Benadjaoud MA
Soysouvanh F
Buard V
Georges T
Vaurijoux A
Gruel G
François A
Guipaud O
Milliat F
Source :
Scientific reports [Sci Rep] 2019 Oct 04; Vol. 9 (1), pp. 14328. Date of Electronic Publication: 2019 Oct 04.
Publication Year :
2019

Abstract

Based on classic clonogenic assay, it is accepted by the scientific community that, whatever the energy, the relative biological effectiveness of X-rays is equal to 1. However, although X-ray beams are widely used in diagnosis, interventional medicine and radiotherapy, comparisons of their energies are scarce. We therefore assessed in vitro the effects of low- and high-energy X-rays using Human umbilical vein endothelial cells (HUVECs) by performing clonogenic assay, measuring viability/mortality, counting γ-H2AX foci, studying cell proliferation and cellular senescence by flow cytometry and by performing gene analysis on custom arrays. Taken together, excepted for γ-H2AX foci counts, these experiments systematically show more adverse effects of high energy X-rays, while the relative biological effectiveness of photons is around 1, whatever the quality of the X-ray beam. These results strongly suggest that multiparametric analysis should be considered in support of clonogenic assay.

Details

Language :
English
ISSN :
2045-2322
Volume :
9
Issue :
1
Database :
MEDLINE
Journal :
Scientific reports
Publication Type :
Academic Journal
Accession number :
31586152
Full Text :
https://doi.org/10.1038/s41598-019-50908-4