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Multiparametric radiobiological assays show that variation of X-ray energy strongly impacts relative biological effectiveness: comparison between 220 kV and 4 MV.
- Source :
-
Scientific reports [Sci Rep] 2019 Oct 04; Vol. 9 (1), pp. 14328. Date of Electronic Publication: 2019 Oct 04. - Publication Year :
- 2019
-
Abstract
- Based on classic clonogenic assay, it is accepted by the scientific community that, whatever the energy, the relative biological effectiveness of X-rays is equal to 1. However, although X-ray beams are widely used in diagnosis, interventional medicine and radiotherapy, comparisons of their energies are scarce. We therefore assessed in vitro the effects of low- and high-energy X-rays using Human umbilical vein endothelial cells (HUVECs) by performing clonogenic assay, measuring viability/mortality, counting γ-H2AX foci, studying cell proliferation and cellular senescence by flow cytometry and by performing gene analysis on custom arrays. Taken together, excepted for γ-H2AX foci counts, these experiments systematically show more adverse effects of high energy X-rays, while the relative biological effectiveness of photons is around 1, whatever the quality of the X-ray beam. These results strongly suggest that multiparametric analysis should be considered in support of clonogenic assay.
- Subjects :
- Cell Survival radiation effects
Colony-Forming Units Assay
DNA Breaks, Double-Stranded radiation effects
Genetic Markers radiation effects
Histones genetics
Human Umbilical Vein Endothelial Cells
Humans
Linear Energy Transfer
Proof of Concept Study
Histones radiation effects
Photons adverse effects
Relative Biological Effectiveness
X-Rays adverse effects
Subjects
Details
- Language :
- English
- ISSN :
- 2045-2322
- Volume :
- 9
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Scientific reports
- Publication Type :
- Academic Journal
- Accession number :
- 31586152
- Full Text :
- https://doi.org/10.1038/s41598-019-50908-4