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Aptamer Induced Multicolored AuNCs-WS 2 "Turn on" FRET Nano Platform for Dual-Color Simultaneous Detection of AflatoxinB 1 and Zearalenone.
- Source :
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Analytical chemistry [Anal Chem] 2019 Nov 05; Vol. 91 (21), pp. 14085-14092. Date of Electronic Publication: 2019 Oct 17. - Publication Year :
- 2019
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Abstract
- Mycotoxins posit serious threats to human and animal health, and numerous efforts have been performed to detect the multiple toxins by a single diagnostic approach. To best of our knowledge, for the first time, we synthesized an aptamer induced "turn on" fluorescence resonance energy transfer (FRET) biosensor using dual-color gold nanoclusters (AuNCs), l-proline, and BSA synthesized AuNCs (Lp-AuNCs and BSA-AuNCs), with WS <subscript>2</subscript> nanosheet for simultaneous recognition of aflatoxinB <subscript>1</subscript> (AFB <subscript>1</subscript> ) and zearalenone (ZEN) by single excitation. Here, AFB <subscript>1</subscript> aptamer stabilized blue-emitting AuNCs (AFB <subscript>1</subscript> -apt-Lp-AuNCs) (at 442 nm) and ZEN aptamer functionalized with red-colored AuNCs (ZEN-apt-BSA-AuNCs) (at 650 nm) were employed as an energy donor and WS <subscript>2</subscript> nanosheet as a fluorescence quencher. With the addition of AFB <subscript>1</subscript> and ZEN, the change in fluorescence intensity (F.I) was recorded at 442 and 650 nm and can be used for simultaneous recognition with a detection limit of 0.34 pg mL <superscript>-1</superscript> ( R <superscript>2</superscript> = 0.9931) and 0.53 pg mL <superscript>-1</superscript> ( R <superscript>2</superscript> = 0.9934), respectively. Most importantly, the semiquantitative determination of AFB <subscript>1</subscript> and ZEN can also be realized through photovisualization. The current approach paves a new way to develop sensitive, selective, and convenient metal nanocluster-based fluorescent "switch-on" probes with potential applications in multipurpose biosensing.
Details
- Language :
- English
- ISSN :
- 1520-6882
- Volume :
- 91
- Issue :
- 21
- Database :
- MEDLINE
- Journal :
- Analytical chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 31585033
- Full Text :
- https://doi.org/10.1021/acs.analchem.9b03880