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Ligand pathways in neuroglobin revealed by low-temperature photodissociation and docking experiments.
- Source :
-
IUCrJ [IUCrJ] 2019 Jul 10; Vol. 6 (Pt 5), pp. 832-842. Date of Electronic Publication: 2019 Jul 10 (Print Publication: 2019). - Publication Year :
- 2019
-
Abstract
- A combined biophysical approach was applied to map gas-docking sites within murine neuroglobin (Ngb), revealing snapshots of events that might govern activity and dynamics in this unique hexacoordinate globin, which is most likely to be involved in gas-sensing in the central nervous system and for which a precise mechanism of action remains to be elucidated. The application of UV-visible microspectroscopy in crystallo , solution X-ray absorption near-edge spectroscopy and X-ray diffraction experiments at 15-40 K provided the structural characterization of an Ngb photolytic intermediate by cryo-trapping and allowed direct observation of the relocation of carbon monoxide within the distal heme pocket after photodissociation. Moreover, X-ray diffraction at 100 K under a high pressure of dioxygen, a physiological ligand of Ngb, unravelled the existence of a storage site for O <subscript>2</subscript> in Ngb which coincides with Xe-III, a previously described docking site for xenon or krypton. Notably, no other secondary sites were observed under our experimental conditions.<br /> (© Chiara Ardiccioni et al. 2019.)
Details
- Language :
- English
- ISSN :
- 2052-2525
- Volume :
- 6
- Issue :
- Pt 5
- Database :
- MEDLINE
- Journal :
- IUCrJ
- Publication Type :
- Academic Journal
- Accession number :
- 31576217
- Full Text :
- https://doi.org/10.1107/S2052252519008157