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Ligand pathways in neuroglobin revealed by low-temperature photodissociation and docking experiments.

Authors :
Ardiccioni C
Arcovito A
Della Longa S
van der Linden P
Bourgeois D
Weik M
Montemiglio LC
Savino C
Avella G
Exertier C
Carpentier P
Prangé T
Brunori M
Colloc'h N
Vallone B
Source :
IUCrJ [IUCrJ] 2019 Jul 10; Vol. 6 (Pt 5), pp. 832-842. Date of Electronic Publication: 2019 Jul 10 (Print Publication: 2019).
Publication Year :
2019

Abstract

A combined biophysical approach was applied to map gas-docking sites within murine neuroglobin (Ngb), revealing snapshots of events that might govern activity and dynamics in this unique hexacoordinate globin, which is most likely to be involved in gas-sensing in the central nervous system and for which a precise mechanism of action remains to be elucidated. The application of UV-visible microspectroscopy in crystallo , solution X-ray absorption near-edge spectroscopy and X-ray diffraction experiments at 15-40 K provided the structural characterization of an Ngb photolytic intermediate by cryo-trapping and allowed direct observation of the relocation of carbon monoxide within the distal heme pocket after photodissociation. Moreover, X-ray diffraction at 100 K under a high pressure of dioxygen, a physiological ligand of Ngb, unravelled the existence of a storage site for O <subscript>2</subscript> in Ngb which coincides with Xe-III, a previously described docking site for xenon or krypton. Notably, no other secondary sites were observed under our experimental conditions.<br /> (© Chiara Ardiccioni et al. 2019.)

Details

Language :
English
ISSN :
2052-2525
Volume :
6
Issue :
Pt 5
Database :
MEDLINE
Journal :
IUCrJ
Publication Type :
Academic Journal
Accession number :
31576217
Full Text :
https://doi.org/10.1107/S2052252519008157