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Calcium Phosphate-Mediated Transfection of Cells with High-Molecular-Weight Genomic DNA.
- Source :
-
Cold Spring Harbor protocols [Cold Spring Harb Protoc] 2019 Oct 01; Vol. 2019 (10). Date of Electronic Publication: 2019 Oct 01. - Publication Year :
- 2019
-
Abstract
- This protocol describes a calcium phosphate-mediated transfection method for use with high-molecular-weight genomic DNAs.<br /> (© 2019 Cold Spring Harbor Laboratory Press.)
- Subjects :
- Animals
Biological Transport drug effects
CHO Cells drug effects
CHO Cells metabolism
Calcium Phosphates chemistry
Calcium Phosphates pharmacology
Cricetinae
Cricetulus
DNA chemistry
DNA metabolism
Genome genetics
Glycerol chemistry
Glycerol metabolism
Glycerol pharmacology
Molecular Weight
Plasmids genetics
Transfection instrumentation
Calcium Phosphates metabolism
DNA genetics
Transfection methods
Subjects
Details
- Language :
- English
- ISSN :
- 1559-6095
- Volume :
- 2019
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- Cold Spring Harbor protocols
- Publication Type :
- Academic Journal
- Accession number :
- 31575794
- Full Text :
- https://doi.org/10.1101/pdb.prot095448