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A Convenient Fluorescence-Based Assay for the Detection of Sucrose Transport and the Introduction of a Sucrose Transporter from Potato into Clostridium Strains.
- Source :
-
Molecules (Basel, Switzerland) [Molecules] 2019 Sep 26; Vol. 24 (19). Date of Electronic Publication: 2019 Sep 26. - Publication Year :
- 2019
-
Abstract
- A convenient and effective sucrose transport assay for Clostridium strains is needed. Traditional methods, such as <superscript>14</superscript> C-sucrose isotope labelling, use radioactive materials and are not convenient for many laboratories. Here, a sucrose transporter from potato was introduced into Clostridium , and a fluorescence assay based on esculin was used for the analysis of sucrose transport in Clostridium strains. This showed that the heterologously expressed potato sucrose transporter is functional in Clostridium . Recombinant engineering of high-level sucrose transport would aid sucrose fermentation in Clostridium strains. The assay described herein provides an important technological platform for studying sucrose transporter function following heterologous expression in Clostridium .
Details
- Language :
- English
- ISSN :
- 1420-3049
- Volume :
- 24
- Issue :
- 19
- Database :
- MEDLINE
- Journal :
- Molecules (Basel, Switzerland)
- Publication Type :
- Academic Journal
- Accession number :
- 31561523
- Full Text :
- https://doi.org/10.3390/molecules24193495