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Dual Site-Specific Labeling of an Antibody Fragment through Sortase A and π-Clamp Conjugation.
- Source :
-
Bioconjugate chemistry [Bioconjug Chem] 2019 Oct 16; Vol. 30 (10), pp. 2539-2543. Date of Electronic Publication: 2019 Oct 03. - Publication Year :
- 2019
-
Abstract
- The functionalization of proteins with different cargo molecules is highly desirable for a broad range of applications. However, the reproducible production of defined conjugates with multiple functionalities is a significant challenge. Herein, we report the dual site-specific labeling of an antibody fragment, utilizing the orthogonal Sortase A and π-clamp conjugation methods, and demonstrate that binding of the antibody fragment to its target receptor is retained after dual labeling.
Details
- Language :
- English
- ISSN :
- 1520-4812
- Volume :
- 30
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- Bioconjugate chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 31560523
- Full Text :
- https://doi.org/10.1021/acs.bioconjchem.9b00639