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How to copy and paste DNA microarrays.
- Source :
-
Scientific reports [Sci Rep] 2019 Sep 26; Vol. 9 (1), pp. 13940. Date of Electronic Publication: 2019 Sep 26. - Publication Year :
- 2019
-
Abstract
- Analogous to a photocopier, we developed a DNA microarray copy technique and were able to copy patterned original DNA microarrays. With this process the appearance of the copied DNA microarray can also be altered compared to the original by producing copies of different resolutions. As a homage to the very first photocopy made by Chester Charlson and Otto Kornei, we performed a lookalike DNA microarray copy exactly 80 years later. Those copies were also used for label-free real-time kinetic binding assays of apo-dCas9 to double stranded DNA and of thrombin to single stranded DNA. Since each DNA microarray copy was made with only 5 µl of spPCR mix, the whole process is cost-efficient. Hence, our DNA microarray copier has a great potential for becoming a standard lab tool.
- Subjects :
- Costs and Cost Analysis
DNA Probes chemistry
DNA Probes genetics
DNA, Single-Stranded chemistry
DNA, Single-Stranded genetics
Humans
Oligonucleotide Array Sequence Analysis economics
Oligonucleotide Array Sequence Analysis instrumentation
Polymerase Chain Reaction economics
Polymerase Chain Reaction instrumentation
Polymerase Chain Reaction methods
Thrombin genetics
Oligonucleotide Array Sequence Analysis methods
Subjects
Details
- Language :
- English
- ISSN :
- 2045-2322
- Volume :
- 9
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Scientific reports
- Publication Type :
- Academic Journal
- Accession number :
- 31558745
- Full Text :
- https://doi.org/10.1038/s41598-019-50371-1