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MiR-599 serves a suppressive role in anaplastic thyroid cancer by activating the T-cell intracellular antigen.
- Source :
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Experimental and therapeutic medicine [Exp Ther Med] 2019 Oct; Vol. 18 (4), pp. 2413-2420. Date of Electronic Publication: 2019 Aug 07. - Publication Year :
- 2019
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Abstract
- Anaplastic thyroid cancer (ATC) has a mean survival time of 6 months and accounts for 1-2% of all thyroid tumors. Understanding the underlying molecular mechanisms of carcinogenesis and progression in ATC would contribute to the identification of novel therapeutic targets. A previous study revealed that microRNA (miR)-599 was associated with tumor initiation and development in certain types of cancer. However, the specific functions and mechanisms of miR-599 in ATC are poorly understood. The objective of the present study was to identify its expression, function and molecular mechanism in ATC. The expression levels of miR-599 in 10 pairs of surgical specimens and human ATC cell lines were examined by reverse transcription-quantitative polymerase chain reaction. Function assays illustrated that miR-599 overexpression not only suppressed KAT-18 cell viability, proliferation and metastasis in vitro and decreased tumor growth in the tumor xenograft model but also induced cell apoptosis. Furthermore, T-cell intracellular antigen (TIA1), a tumor suppressor, was confirmed as a direct target of miR-599. It was demonstrated that TIA1 silencing rescued the inhibitory effect of migration and invasion induced by the overexpression of miR-599 in KAT-18 cells. In conclusion, the present study revealed that miR-599 inhibited ATC cell growth and metastasis via activation of TIA1. Therefore miR-599 may be a novel molecular therapeutic target for ATC.
Details
- Language :
- English
- ISSN :
- 1792-0981
- Volume :
- 18
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Experimental and therapeutic medicine
- Publication Type :
- Academic Journal
- Accession number :
- 31555352
- Full Text :
- https://doi.org/10.3892/etm.2019.7864