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Krüppel-like factor KLF9 inhibits chicken intramuscular preadipocyte differentiation.
- Source :
-
British poultry science [Br Poult Sci] 2019 Dec; Vol. 60 (6), pp. 790-797. Date of Electronic Publication: 2019 Sep 23. - Publication Year :
- 2019
-
Abstract
- 1. Poultry meat quality is affected by many factors, among which intramuscular fat (IMF) is predominant. IMF content affects tenderness, juiciness and flavour of meat. Krüppel-like transcriptional factors (KLFs) are important regulators of adipocyte differentiation. However, little is known about the KLF9 gene associated with poultry IMF deposition, especially intramuscular adipocyte differentiation.2. Previous work has shown that chicken KLF9 was differentially expressed during adipogenesis of intramuscular preadipocytes differentiation. In this study, the function of KLF9 in chicken intramuscular preadipocytes differentiation was investigated.3. In the chicken preadipocyte differentiation model, KLF9 expression showed a major increase with adipogenic induction. Overexpression of KLF9 down-regulated the expression of the adipogenic marker gene AP2 , and impaired triglyceride accumulation. Knockdown of KLF9 in chicken intramuscular preadipocytes increased the expression of PPARG, CEBPA and AP2 . In addition, it was proposed that KLF9 may regulate adipogenesis via lncRNAs NONGGAT002209.2, NONGGAT003346.2, NONGGAT000436.2 and NONGGAT006302.2 in chicken.4. The data supported a novel role of KLF9 in regulating chicken intramuscular preadipocyte differentiation. Such findings may contribute to a more thorough understanding of chicken IMF deposition and the improvement of poultry meat quality.
- Subjects :
- Adipocytes metabolism
Adipose Tissue cytology
Amino Acid Sequence
Analysis of Variance
Animals
Azo Compounds
Base Sequence
Cell Differentiation
Cells, Cultured
Coloring Agents
Kruppel-Like Transcription Factors chemistry
Kruppel-Like Transcription Factors classification
Kruppel-Like Transcription Factors pharmacology
Meat standards
Pectoralis Muscles cytology
Pectoralis Muscles growth & development
Pectoralis Muscles metabolism
Phylogeny
Plasmids genetics
RNA, Messenger chemistry
RNA, Messenger isolation & purification
Real-Time Polymerase Chain Reaction veterinary
Sequence Alignment veterinary
Staining and Labeling veterinary
Transfection veterinary
Adipocytes cytology
Chickens physiology
Kruppel-Like Transcription Factors physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1466-1799
- Volume :
- 60
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- British poultry science
- Publication Type :
- Academic Journal
- Accession number :
- 31542936
- Full Text :
- https://doi.org/10.1080/00071668.2019.1657229