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Shiga Toxin Induces Lipid Compression: A Mechanism for Generating Membrane Curvature.
- Source :
-
Nano letters [Nano Lett] 2019 Oct 09; Vol. 19 (10), pp. 7365-7369. Date of Electronic Publication: 2019 Sep 25. - Publication Year :
- 2019
-
Abstract
- Biomembranes are hard to compress laterally, and membrane area compressibility has not been associated with biological processes. Using X-ray surface scattering, we observed that bacterial Shiga toxin compresses lipid packing in a gel phase monolayer upon binding to its cellular receptor, the glycolipid Gb3. This toxin-induced reorganization of lipid packing reached beyond the immediate membrane patch that the protein was bound to, and linkers separating the Gb3 carbohydrate and ceramide moieties modulated the toxin's capacity to compress the membrane. Within a natural membrane, asymmetric compression of the toxin-bound leaflet could provide a mechanism to initiate narrow membrane bending, as observed upon toxin entry into cells. Such lipid compression and long-range membrane reorganization by glycolipid-binding proteins represent novel concepts in membrane biology that have direct implications for the construction of endocytic pits in clathrin-independent endocytosis.
Details
- Language :
- English
- ISSN :
- 1530-6992
- Volume :
- 19
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- Nano letters
- Publication Type :
- Academic Journal
- Accession number :
- 31538793
- Full Text :
- https://doi.org/10.1021/acs.nanolett.9b03001