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β-Glucosidase BGL1 from Coprinopsis cinerea Exhibits a Distinctive Hydrolysis and Transglycosylation Activity for Application in the Production of 3-O-β-d-Gentiobiosyl-d-laminarioligosaccharides.
- Source :
-
Journal of agricultural and food chemistry [J Agric Food Chem] 2019 Sep 25; Vol. 67 (38), pp. 10744-10755. Date of Electronic Publication: 2019 Sep 17. - Publication Year :
- 2019
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Abstract
- We previously reported that β-glucosidase BGL1 at low concentration (15 μg mL <superscript>-1</superscript> ) from Coprinopsis cinerea exhibited hydrolytic activity only toward laminarioligosaccharides but not toward cellooligosaccharides and gentiobiose. This study shows that BGL1 at high concentration (200 μg mL <superscript>-1</superscript> ) also hydrolyzed cellobiose and gentiobiose, which accounted for only 0.83 and 2.05% of its activity toward laminaribiose, respectively. Interestingly, BGL1 at low concentration (1.5 μg mL <superscript>-1</superscript> ) showed transglycosylation but BGL1 at high concentration (200 μg mL <superscript>-1</superscript> ) did not. BGL1 utilizes only laminarioligosaccharides but not glucose, gentiobiose, and cellobiose to synthesize the higher oligosaccharides. BGL1 transferred one glucosyl residue from substrate laminarioligosaccharide to another laminarioligosaccharide as an acceptor in a β(1 → 3) or β(1 → 6) fashion to produce higher laminarioligosaccharides or 3-O-β-d-gentiobiosyl-d-laminarioligosaccharides. The BGL1-digested laminaritriose exhibited approximately 90% enhancement in the anti-oxidant activity compared to that of untreated laminaritriose, implying a potential application of BGL1-based transglycosylation for the production of high value-added rare oligosaccharides.
- Subjects :
- Agaricales chemistry
Agaricales genetics
Amino Acid Sequence
Disaccharides chemistry
Fungal Proteins genetics
Fungal Proteins metabolism
Glycosylation
Hydrolysis
Kinetics
Molecular Structure
Oligosaccharides chemistry
Substrate Specificity
beta-Glucosidase genetics
beta-Glucosidase metabolism
Agaricales enzymology
Disaccharides metabolism
Fungal Proteins chemistry
Oligosaccharides metabolism
beta-Glucosidase chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1520-5118
- Volume :
- 67
- Issue :
- 38
- Database :
- MEDLINE
- Journal :
- Journal of agricultural and food chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 31525900
- Full Text :
- https://doi.org/10.1021/acs.jafc.9b04488