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Recognition of Schistosoma mansoni egg-expressed ovalbumin by T cell receptor transgenic mice.
- Source :
-
Experimental parasitology [Exp Parasitol] 2019 Nov; Vol. 206, pp. 107767. Date of Electronic Publication: 2019 Sep 11. - Publication Year :
- 2019
-
Abstract
- Schistosoma mansoni eggs can influence immune responses directed at them, and the mechanisms by which this is achieved are being unravelled. Going towards, developing effective tools for the study of how S. mansoni influences naïve T cells, we have developed S. mansoni eggs expressing chicken ovalbumin (OVA), using a lentiviral transduction system. Indeed, such a parasite could be used in conjunction with cells from OT-II transgenic mice as a source of naïve, antigen-specific T cells. The expression of the transgenic protein was confirmed by real-time RT-PCR of OVA-specific mRNA and western blotting using polyclonal antibodies specific for OVA. T cells from OT-II transgenic mice expressing a T cell receptor specific for the OVA <subscript>323-339</subscript> peptide recognised the OVA-transduced S. mansoni eggs. Using flow cytometry on CFSE-labelled OT-II splenocytes, we demonstrated that OVA-transduced eggs elicit higher OT-II proliferative responses than untransduced eggs. The OT-II T cells also produced TNF-α and IFN-γ following exposure to OVA-transduced eggs. In addition, moderate amounts of IL-6 and IL-17A were also detected. In contrast, no IL-10, IL-4 and IL-2 were detected in cultures, whether the cells were stimulated with transduced or untransduced eggs. Thus, the cytokine signatures showed the transfected eggs induced predominantly a Th1 response, with a small amount of IL-6 and IL-17.<br /> (Copyright © 2019 Elsevier Inc. All rights reserved.)
- Subjects :
- Animals
Blotting, Western
Chickens
Cytokines analysis
Cytokines metabolism
Electrophoresis, Agar Gel
Female
Flow Cytometry
Interleukin-17 analysis
Interleukin-17 metabolism
Interleukin-2 analysis
Interleukin-2 metabolism
Interleukin-6 analysis
Interleukin-6 metabolism
Liver parasitology
Lymph Nodes cytology
Mice
Mice, Inbred BALB C
Mice, Transgenic
Ovalbumin genetics
Ovalbumin immunology
Ovalbumin metabolism
Ovum metabolism
RNA, Messenger analysis
RNA, Messenger isolation & purification
Real-Time Polymerase Chain Reaction
Receptors, Antigen, T-Cell genetics
Reverse Transcription
Schistosoma mansoni genetics
Schistosoma mansoni growth & development
Spleen cytology
T-Lymphocytes cytology
Ovalbumin analysis
Receptors, Antigen, T-Cell immunology
Schistosoma mansoni metabolism
T-Lymphocytes immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1090-2449
- Volume :
- 206
- Database :
- MEDLINE
- Journal :
- Experimental parasitology
- Publication Type :
- Academic Journal
- Accession number :
- 31520603
- Full Text :
- https://doi.org/10.1016/j.exppara.2019.107767