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Feasibility of flow cytometric analysis of restricted light chain in endoscopic biopsy specimens from patients with gastrointestinal tract B cell lymphoma: a pilot study.
- Source :
-
BMC research notes [BMC Res Notes] 2019 Sep 11; Vol. 12 (1), pp. 571. Date of Electronic Publication: 2019 Sep 11. - Publication Year :
- 2019
-
Abstract
- Objective: Gastrointestinal tract lymphomas are currently detected more frequently due to advances in endoscopic technology. The aim of this study was to assess the feasibility of flow cytometric analysis of restricted light chain in endoscopic biopsy specimens for the diagnosis of gastrointestinal tract B-cell lymphoma. We prepared viable cell suspensions from unfixed specimens obtained from 10 consecutive patients who had a previous histological diagnosis of gastrointestinal tract B-cell lymphoma. We performed immunophenotypic studies with multi-color flow cytometry and assessed clonality through examination of immunoglobulin light chain expression exclusively in a population identified by anti-CD45 or CD20 antibodies.<br />Results: We could perform light chain expression analysis with 2 endoscopic biopsy specimens from all 10 patients with gastrointestinal tract B-cell lymphoma. We conclude that flow cytometric analysis of endoscopic biopsy specimens is feasible and thus likely useful for the diagnosis of gastrointestinal tract B-cell lymphoma in clinical settings. Trial registration UMIN Clinical Trials Registry, UMIN000027730. Registered 12 June 2017.
- Subjects :
- Aged
Aged, 80 and over
B-Lymphocytes immunology
B-Lymphocytes metabolism
B-Lymphocytes pathology
Feasibility Studies
Female
Gastrointestinal Neoplasms metabolism
Gastrointestinal Neoplasms pathology
Humans
Immunoglobulin Light Chains immunology
Immunophenotyping
Lymphoma, B-Cell metabolism
Lymphoma, B-Cell pathology
Male
Middle Aged
Pilot Projects
Endoscopic Ultrasound-Guided Fine Needle Aspiration methods
Flow Cytometry methods
Gastrointestinal Neoplasms immunology
Immunoglobulin Light Chains analysis
Lymphoma, B-Cell immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1756-0500
- Volume :
- 12
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- BMC research notes
- Publication Type :
- Academic Journal
- Accession number :
- 31511043
- Full Text :
- https://doi.org/10.1186/s13104-019-4578-4