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Express ELISA for detection of mortalin.
- Source :
-
BioTechniques [Biotechniques] 2019 Oct; Vol. 67 (4), pp. 166-171. Date of Electronic Publication: 2019 Sep 10. - Publication Year :
- 2019
-
Abstract
- Mortalin is a widely studied stress chaperone that plays a significant role in diseases such as cancer, diabetes mellitus, liver cirrhosis, neurodegeneration and generalized aging. Based on these, the level of mortalin expression has been predicted to be an important and valuable diagnostic and prognostic marker. Conventional methods of protein analyses, such as Western blotting, immunohistochemistry or ELISA with antibodies provide specific, sensitive and useful outcomes. However, they are limited by lengthy and time-consuming protocols. Here, we present an upgrade to the existing ELISA techniques. We have prepared a conjugate of anti-mortalin antibody and luciferase enzyme that can be recruited for rapid (∼3 h) and quantitative detection of mortalin expression in a given biological sample.
- Subjects :
- A549 Cells
Animals
Antibodies, Monoclonal genetics
Blotting, Western
Cell Line
Goats
HSP70 Heat-Shock Proteins immunology
Humans
Luciferases genetics
Mice
Mitochondrial Proteins immunology
Sensitivity and Specificity
Enzyme-Linked Immunosorbent Assay methods
HSP70 Heat-Shock Proteins analysis
Mitochondrial Proteins analysis
Subjects
Details
- Language :
- English
- ISSN :
- 1940-9818
- Volume :
- 67
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- BioTechniques
- Publication Type :
- Academic Journal
- Accession number :
- 31502469
- Full Text :
- https://doi.org/10.2144/btn-2018-0158