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The effects of sympathetic nerve damage on satellite glial cells in the mouse superior cervical ganglion.
- Source :
-
Autonomic neuroscience : basic & clinical [Auton Neurosci] 2019 Nov; Vol. 221, pp. 102584. Date of Electronic Publication: 2019 Aug 24. - Publication Year :
- 2019
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Abstract
- Neurons in sensory, sympathetic, and parasympathetic ganglia are surrounded by satellite glial cell (SGCs). There is little information on the effects of nerve damage on SGCs in autonomic ganglia. We studied the consequences of damage to sympathetic nerve terminals by 6-hydroxydopamine (6-OHDA) on SGCs in the mouse superior cervical ganglia (Sup-CG). Immunostaining revealed that at 1-30 d post-6-OHDA injection, SGCs in Sup-CG were activated, as assayed by upregulation of glial fibrillary acidic protein. Intracellular labeling showed that dye coupling between SGCs around different neurons increased 4-6-fold 1-14 d after 6-OHDA injection. Behavioral testing 1-7 d post-6-OHDA showed that withdrawal threshold to tactile stimulation of the hind paws was reduced by 65-85%, consistent with hypersensitivity. A single intraperitoneal injection of the gap junction blocker carbenoxolone restored normal tactile thresholds in 6-OHDA-treated mice, suggesting a contribution of SGC gap junctions to pain. Using calcium imaging we found that after 6-OHDA treatment responses of SGCs to ATP were increased by about 30% compared with controls, but responses to ACh were reduced by 48%. The same experiments for SGCs in trigeminal ganglia from 6-OHDA injected mice showed no difference from controls, confirming that 6-OHDA acted selectively on sympathetic nerves. However, systemic inflammation induced by lipopolysaccharide did not affect SGCs of Sup-CG, but did influence SGCs in trigeminal ganglia in the same manner as 6-OHDA did on SGCs in Sup-CG. We conclude that even though SGCs in sympathetic and sensory ganglia are morphologically similar, they are quite different functionally, particularly after damage.<br /> (Copyright © 2019. Published by Elsevier B.V.)
- Subjects :
- Acetylcholine pharmacology
Adenosine Triphosphate pharmacology
Animals
Calcium Signaling
Carbenoxolone pharmacology
Cell Communication drug effects
Female
Gap Junctions drug effects
Gap Junctions physiology
Glutamate-Ammonia Ligase biosynthesis
Glutamate-Ammonia Ligase genetics
Lipopolysaccharides toxicity
Male
Mice
Mice, Inbred BALB C
Nerve Tissue Proteins biosynthesis
Nerve Tissue Proteins genetics
Neuralgia physiopathology
Oxidopamine toxicity
Pain Threshold physiology
Satellite Cells, Perineuronal drug effects
Toll-Like Receptor 4 biosynthesis
Toll-Like Receptor 4 genetics
Trigeminal Ganglion pathology
Satellite Cells, Perineuronal physiology
Superior Cervical Ganglion pathology
Sympathetic Nervous System drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 1872-7484
- Volume :
- 221
- Database :
- MEDLINE
- Journal :
- Autonomic neuroscience : basic & clinical
- Publication Type :
- Academic Journal
- Accession number :
- 31494528
- Full Text :
- https://doi.org/10.1016/j.autneu.2019.102584