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Updating Molecular Diagnostics for Detecting Methicillin-Susceptible and Methicillin-Resistant Staphylococcus aureus Isolates in Blood Culture Bottles.
- Source :
-
Journal of clinical microbiology [J Clin Microbiol] 2019 Oct 23; Vol. 57 (11). Date of Electronic Publication: 2019 Oct 23 (Print Publication: 2019). - Publication Year :
- 2019
-
Abstract
- Molecular diagnostic tests can be used to provide rapid identification of staphylococcal species in blood culture bottles to help improve antimicrobial stewardship. However, alterations in the target nucleic acid sequences of the microorganisms or their antimicrobial resistance genes can lead to false-negative results. We determined the whole-genome sequences of 4 blood culture isolates of Staphylococcus aureus and 2 control organisms to understand the genetic basis of genotype-phenotype discrepancies when using the Xpert MRSA/SA BC test ( in vitro diagnostic medical device [IVD]). Three methicillin-resistant S. aureus (MRSA) isolates each had a different insertion of a genetic element in the staphylococcal cassette chromosome (SCC mec )- orfX junction region that led to a misclassification as methicillin-susceptible S. aureus (MSSA). One strain contained a deletion in spa , which produced a false S. aureus -negative result. A control strain of S. aureus that harbored an SCC mec element but no mecA (an empty cassette) was correctly called MSSA by the Xpert test. The second control contained an SCC <subscript>M1</subscript> insertion. The updated Xpert MRSA/SA BC test successfully detected both spa and SCC mec variants of MRSA and correctly identified empty-cassette strains of S. aureus as MSSA. Among a sample of 252 MSSA isolates from the United States and Europe, 3.9% contained empty SCC mec cassettes, 1.6% carried SCC <subscript>M1</subscript> , <1% had spa deletions, and <1% contained SCC mec variants other than those with SCC <subscript>M1</subscript> These data suggest that genetic variations that may interfere with Xpert MRSA/SA BC test results remain rare. Results for all the isolates were correct when tested with the updated assay.<br /> (Copyright © 2019 Tenover et al.)
- Subjects :
- Anti-Bacterial Agents pharmacology
DNA, Bacterial genetics
False Negative Reactions
Genetic Variation
Genotype
Humans
Methicillin pharmacology
Methicillin-Resistant Staphylococcus aureus drug effects
Microbial Sensitivity Tests
Molecular Diagnostic Techniques methods
Phenotype
Staphylococcal Infections microbiology
Staphylococcus aureus drug effects
Whole Genome Sequencing
Bacterial Proteins genetics
Blood Culture methods
Methicillin-Resistant Staphylococcus aureus genetics
Molecular Diagnostic Techniques standards
Staphylococcal Infections blood
Staphylococcus aureus genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1098-660X
- Volume :
- 57
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- Journal of clinical microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 31484703
- Full Text :
- https://doi.org/10.1128/JCM.01195-19