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Aptamer Selection for Detecting Molecular Target Using Cell-SELEX (Systematic Evolution of Ligands by Exponential Enrichment) Technology.
- Source :
-
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2019; Vol. 2054, pp. 223-241. - Publication Year :
- 2019
-
Abstract
- Cell-SELEX is a live cell-based in vitro selection method that generates functional oligonucleotides, or aptamers. Often referenced as the chemist's antibody, aptamers bind to select targets with high affinity and can be utilized in a number of applications, including biomedicine, bioimaging, and biosensing. Here we describe the cell-SELEX technique and discuss this methodology's unique merit(s)-namely the ability to isolate highly selective aptamer panels with no prior knowledge of cellular signatures. This strategy thus presents as a technology that has the potential to enhance the precision of molecular medicine and targeted therapeutics.
- Subjects :
- Aptamers, Nucleotide genetics
Aptamers, Nucleotide pharmacology
Cell Culture Techniques methods
Cell Line
DNA, Single-Stranded genetics
DNA, Single-Stranded pharmacology
Humans
Molecular Targeted Therapy methods
Precision Medicine methods
SELEX Aptamer Technique instrumentation
Theranostic Nanomedicine methods
Aptamers, Nucleotide isolation & purification
DNA, Single-Stranded isolation & purification
SELEX Aptamer Technique methods
Subjects
Details
- Language :
- English
- ISSN :
- 1940-6029
- Volume :
- 2054
- Database :
- MEDLINE
- Journal :
- Methods in molecular biology (Clifton, N.J.)
- Publication Type :
- Academic Journal
- Accession number :
- 31482459
- Full Text :
- https://doi.org/10.1007/978-1-4939-9769-5_15