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Dysregulation of miR-204-3p Driven by the Viability and Motility of Retinoblastoma via Wnt/β-catenin Pathway In Vitro and In Vivo.
- Source :
-
Pathology oncology research : POR [Pathol Oncol Res] 2020 Jul; Vol. 26 (3), pp. 1549-1558. Date of Electronic Publication: 2019 Sep 03. - Publication Year :
- 2020
-
Abstract
- Retinoblastoma (RB) is a malignant intraocular tumor that frequently occurs in infants and toddlers. Although the most of RB patients in the developed countries could survival from this cancer, the patients in undeveloped areas are still suffering. The human retinal pigment epithelial cell line ARPE-19 and human retinoblastoma (RB) cell lines HXO-RB44, Y79, and WERI-Rb1 were cultured. The mRNA levels of BANCR and miR-204-3p in these cell lines were measured by qRT-PCR. After transfection with sh-BANCR or treatment with miR-204-3p inhibitor in Y79 cells, the cell proliferation rate, growth, invasion, migration, apoptosis and Wnt/β-catenin signaling pathway activity were measured. The regular Y79 and Y79 cells stably expressed sh-BANCR were injected subcutaneously into nude mice, respectively. The volumes and pathohistological futures of tumors were compared. The biochemical features similar to the cell culture were detected and compered. The mRNA measurements showed that BANCR negatively modulate miR-204-3p expression via directly integration with it. Besides, miR-204-3p and Wnt/β-catenin signalling pathway were found to participate in the oncogenic effects of BANCR on RB cell line by Hoechst staining, cell Counting Kit-8 (CCK-8) assay, wound healing assay, transwell assay, and Western blot analysis in vitro. In addition, an in vivo tumorigenesis experiment in nude mice injected with Y79 cells stably expressed sh-BANCR conformed in the effects of BANCR on RB. Taken together, the knockdown of BANCR inhibited cell proliferation, apoptosis, invasion, and migration in RB via targeting miR-204-3p, the mechanism may involve inhibiting Wnt/β-catenin signaling pathway.
- Subjects :
- Animals
Apoptosis genetics
Cell Line, Tumor
Cell Movement genetics
Cell Proliferation genetics
Cell Survival genetics
Heterografts
Humans
Mice
Mice, Nude
Neoplasm Invasiveness genetics
Retinal Neoplasms genetics
Retinoblastoma genetics
Gene Expression Regulation, Neoplastic genetics
MicroRNAs genetics
RNA, Long Noncoding genetics
Retinal Neoplasms pathology
Retinoblastoma pathology
Wnt Signaling Pathway genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1532-2807
- Volume :
- 26
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Pathology oncology research : POR
- Publication Type :
- Academic Journal
- Accession number :
- 31482398
- Full Text :
- https://doi.org/10.1007/s12253-019-00722-0