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A triple-amplification differential pulse voltammetry for sensitive detection of DNA based on exonuclease III, strand displacement reaction and terminal deoxynucleotidyl transferase.
- Source :
-
Biosensors & bioelectronics [Biosens Bioelectron] 2019 Oct 15; Vol. 143, pp. 111609. Date of Electronic Publication: 2019 Aug 22. - Publication Year :
- 2019
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Abstract
- In this research, a sensitive and specific electrochemical biosensor for DNA detection was constructed. The highly sensitivity of this biosensor is due to the exploitation of exonuclease III-assisted double recycling and toehold-mediated strand displacement recycling to achieve the target triple recycling amplification, thus generating a large amount of Y-shaped DNA structures. Combination with a terminal deoxynucleotidyl transferase (TDT)-mediated cascaded signal amplification strategy can catalyze the repetitive incorporation of biotin-dUTP to the 3'-OH of the Y-shaped DNA. Via biotin-streptavidin interaction, multiple streptavidin-alkaline phosphatases were conjugated to the surface of an Au electrode and generated a sharply increasing electrochemical signal in a 1-naphthyl phosphate (1-NP) solution. In this method, an impressive detection limit of 0.05 fM was obtained, presenting outstanding selectivity with a dynamic response scope between 0.1 fM and 1 nΜ. Thus, the designed biosensor opens an avenue for DNA detection in clinical molecular diagnostics, pathogen detection, gene therapy, food safety and environmental monitoring.<br /> (Copyright © 2019 Elsevier B.V. All rights reserved.)
- Subjects :
- Alkaline Phosphatase chemistry
Biotin chemistry
Catalysis
DNA chemistry
Electrodes
Exodeoxyribonucleases chemistry
Limit of Detection
Nucleic Acid Amplification Techniques
Streptavidin chemistry
Biosensing Techniques
DNA isolation & purification
DNA Nucleotidylexotransferase chemistry
Electrochemical Techniques
Subjects
Details
- Language :
- English
- ISSN :
- 1873-4235
- Volume :
- 143
- Database :
- MEDLINE
- Journal :
- Biosensors & bioelectronics
- Publication Type :
- Academic Journal
- Accession number :
- 31476597
- Full Text :
- https://doi.org/10.1016/j.bios.2019.111609