Dose-specific biochemical and erythrocytic alterations of anthracene exposure on blood of Anabas testudineus (Bloch).

The present work is designed to compare the chronic toxicity of anthracene [one of the major constituents of polycyclic aromatic hydrocarbons (PAHs)] on Anabas testudineus (Bloch), in an air-breathing carnivorous fish, in laboratory condition under the exposure of two doses of LC ₅₀ value, i.e., 0.0075 mg/l (T1), i.e., 25% and 0.015 mg/l (T2) i.e., 50% for 21 days. A comprehensive comparison was recorded based on biochemical parameters and evaluated the erythrocytic alterations of blood components of the fish. It revealed an enhanced trend of activity of glutamic pyruvic transamin (GPT) 470.7 ± 12.32, 546.6 ± 13.22, 599.4 ± 13.09 U/L and alkaline phosphatase (ALP) 9.2 ± 0.61, 10.4 ± 0.86, 10.9 ± 0.74 U/L in control, T1 and T2 respectively; and reverse trend of protein (PRO) 26.63 ± 1.32, 22.15 ± 1.13, 22.29 ± 1.02 g/dl and albumin (ALB) 11.9 ± 0.71, 9.65 ± 0.91, 10.05 ± 0.94 g/dl in control, T1 and T2 respectively. Under T1 and T2 exposure conditions, it displayed the maximum alterations and appearance of tear drop-like cells (Tr), sickle cells (Sk), swelled cells (Sc) and vacuolated cells (Va) in comparison to control condition. An exclusive experimentation of the present work suggested that biochemical parameters and erythrocytic alterations may be useful tool as biomarkers to monitor the long term toxicological effects, especially to anthracene a constituent of PAHs, in any aquatic environment.
(Copyright © 2019. Published by Elsevier B.V.)