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Guggulipid ameliorates adjuvant-induced arthritis and liver oxidative damage by suppressing inflammatory and oxidative stress mediators.
- Source :
-
Phytomedicine : international journal of phytotherapy and phytopharmacology [Phytomedicine] 2019 Nov; Vol. 64, pp. 152924. Date of Electronic Publication: 2019 Apr 09. - Publication Year :
- 2019
-
Abstract
- Background: Arthritis is a common degenerative joint disease characterized by deterioration of articular cartilage, subchondral bone, and associated with immobility, pain and inflammation. The incessant action of reactive oxygen species (ROS) during progressive arthritis causes severe oxidative damage to vital organs and circulatory system.<br />Purpose: In this study we investigated the ability of guggulipid (GL), a lipid rich extract from the gum resin of the plant Commiphora whighitii to suppress the progressive arthritis and associated liver oxidative stress both in vivo and in vitro.<br />Study Design/methods: The anti-arthritic ability of GL was demonstrated in vitro using IL-1β stimulated bovine nasal cartilage model and in vivo Freund's complete adjuvant-induced arthritic rat model. Collagen/proteoglycan degradation and pro-inflammatory mediators were monitored in the harvested culture medium of nasal cartilage by estimating the levels of matrix metalloproteinases (MMPs), hydroxy proline, glycosaminoglycans and inflammatory mediators. Further, anti-arthritic ability of GL was evaluated in vivo by measuring enzymatic and non-enzymatic mediators of cartilage degradation, inflammation and oxidative stress markers.<br />Results: GL significantly inhibited the IL-1β stimulated cartilage degradation in vitro by mitigating the MMPs activity, collagen degradation and secretion of pro-inflammatory mediators. Further, GL significantly reduced the adjuvant-induced paw swelling and body weight loss in vivo. GL remarkably reduced the MMPs and hyaluronidases activities in serum and bone homogenate along with altered hematological parameters. GL also mitigated the elevated bone resorbing enzymes cathepsins, exoglycosidases and phosphatases. Additionally, GL effectively mitigated ROS and oxidative stress-mediators recuperating the altered serum/liver oxidative stress and liver damage incurred during arthritic progression.<br />Conclusion: In summary, the study clearly demonstrates the protective efficacy of GL against arthritis and its associated oxidative stress, particularly, liver oxidative damage. Hence, GL could be a potential alternative and complementary medicine to treat inflammatory joint diseases.<br /> (Copyright © 2019 Elsevier GmbH. All rights reserved.)
- Subjects :
- Animals
Anti-Inflammatory Agents administration & dosage
Arthritis, Experimental chemically induced
Cattle
Disease Models, Animal
Freund's Adjuvant adverse effects
Inflammation Mediators metabolism
Interleukin-1beta metabolism
Liver drug effects
Nasal Cartilages drug effects
Plant Extracts administration & dosage
Plant Gums administration & dosage
Rats
Rats, Wistar
Reactive Oxygen Species metabolism
Anti-Inflammatory Agents pharmacology
Arthritis, Experimental drug therapy
Commiphora chemistry
Inflammation drug therapy
Oxidative Stress drug effects
Plant Extracts pharmacology
Plant Gums pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1618-095X
- Volume :
- 64
- Database :
- MEDLINE
- Journal :
- Phytomedicine : international journal of phytotherapy and phytopharmacology
- Publication Type :
- Academic Journal
- Accession number :
- 31465983
- Full Text :
- https://doi.org/10.1016/j.phymed.2019.152924