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Protective effects of raspberry on the oxidative damage in HepG2 cells through Keap1/Nrf2-dependent signaling pathway.
- Source :
-
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association [Food Chem Toxicol] 2019 Nov; Vol. 133, pp. 110781. Date of Electronic Publication: 2019 Aug 26. - Publication Year :
- 2019
-
Abstract
- The aim of the present study was to explore the protective effects of raspberry and its bioactive compound cyanidin 3-O-glucoside against H <subscript>2</subscript> O <subscript>2</subscript> -induced oxidative stress in HepG2 cells. We established a model of oxidative stress in HepG2 cells induced by H <subscript>2</subscript> O <subscript>2</subscript> and examined the protein expression of Keap1/Nrf2. The antioxidant activity of raspberry extract was carried out measuring the level of reactive oxygen species (ROS), and the changes of phase II detoxification elements such as GSH level and CAT activity. Also the expression of proteins related to the Keap1/Nrf2 signaling was tested. The results revealed that raspberry extract significantly reduced the ROS levels in oxidative injured cells, increased GSH content and CAT activity, and activated the expression of proteins Keap1, Nrf2, HO-1, NQO1, and γ-GCS. These results taken together indicated that raspberry treatment could ameliorate H <subscript>2</subscript> O <subscript>2</subscript> -induced oxidative stress in HepG2 cells via Keap1/Nrf2 pathway.<br /> (Copyright © 2019 Elsevier Ltd. All rights reserved.)
- Subjects :
- Anthocyanins pharmacology
Fruit chemistry
Glucosides pharmacology
Hep G2 Cells
Humans
Hydrogen Peroxide pharmacology
Rubus chemistry
Antioxidants pharmacology
Kelch-Like ECH-Associated Protein 1 metabolism
NF-E2-Related Factor 2 metabolism
Oxidative Stress drug effects
Plant Extracts pharmacology
Signal Transduction drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 1873-6351
- Volume :
- 133
- Database :
- MEDLINE
- Journal :
- Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association
- Publication Type :
- Academic Journal
- Accession number :
- 31465820
- Full Text :
- https://doi.org/10.1016/j.fct.2019.110781