Quantitative discrimination of biological tissues by micro-elastographic measurement using an epi-illumination Mueller matrix microscope.

Authors :: Mukherjee P
Horiguchi T
Shibata S
Hagen N
Otani Y
Source :: Biomedical optics express [Biomed Opt Express] 2019 Jul 09; Vol. 10 (8), pp. 3847-3859. Date of Electronic Publication: 2019 Jul 09 (Print Publication: 2019).
Publication Year :: 2019
Abstract: We propose a method for estimating the stiffness of bio-specimens by measuring their linear retardance properties under applied stress. For this purpose, we employ an epi-illumination Mueller matrix microscope and show the procedures for its calibration. We provide experimental results demonstrating how to apply Mueller matrix data to elastography, using chicken liver and chicken heart as biological samples. Finally, we show how the histograms of linear retardance images can be used to distinguish between specimens and quantify the discrimination accuracy.<br />Competing Interests: The authors declare that there are no conflicts of interest related to this article.

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