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Detection of Cryptosporidium Recovered from Large-Volume Water Samples Using Dead-End Ultrafiltration.
- Source :
-
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2020; Vol. 2052, pp. 23-41. - Publication Year :
- 2020
-
Abstract
- The procedure described here provides instructions for detection of Cryptosporidium recovered from large-volume water samples. Water samples are collected by dead-end ultrafiltration in the field and ultrafilters are processed in a laboratory. Microbes recovered from the filters are further concentrated and subjected to Cryptosporidium isolation or nucleic acid extraction methods for the detection of Cryptosporidium oocysts or Cryptosporidium DNA.
- Subjects :
- Cryptosporidium genetics
DNA, Protozoan genetics
DNA, Protozoan isolation & purification
Fluorescent Antibody Technique methods
Microscopy, Fluorescence instrumentation
Oocysts immunology
Ultrafiltration instrumentation
Workflow
Cryptosporidium isolation & purification
Microscopy, Fluorescence methods
Real-Time Polymerase Chain Reaction methods
Ultrafiltration methods
Water parasitology
Subjects
Details
- Language :
- English
- ISSN :
- 1940-6029
- Volume :
- 2052
- Database :
- MEDLINE
- Journal :
- Methods in molecular biology (Clifton, N.J.)
- Publication Type :
- Academic Journal
- Accession number :
- 31452155
- Full Text :
- https://doi.org/10.1007/978-1-4939-9748-0_3