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Helicobacter pylori lipids can form ordered membrane domains (rafts).
- Source :
-
Biochimica et biophysica acta. Biomembranes [Biochim Biophys Acta Biomembr] 2019 Nov 01; Vol. 1861 (11), pp. 183050. Date of Electronic Publication: 2019 Aug 23. - Publication Year :
- 2019
-
Abstract
- Ordered lipid domains (rafts) are generally considered to be features of eukaryotic cells, but ordered lipid domains formed by cholesterol lipids have been identified in bacteria from the genus Borrelia, and similar cholesterol lipids exist in the bacterium Helicobacter pylori. To determine whether H. pylori lipids could form ordered membrane domains, we investigated domain formation in aqueous dispersions of H. pylori whole lipid extracts, individual H. pylori lipids, or defined mixtures of H. pylori lipids and other membrane-forming lipids. DPH (1,6-diphenyl-1,3,5-hexatriene) anisotropy measurements were used to assay membrane order and FRET (Förster resonance energy transfer) was used to detect the presence of co-existing ordered and disordered domains. We found that H. pylori membrane lipid extracts spontaneously formed lipid domains. Domain formation was more stable when lipids were extracted from H. pylori cells grown in the presence of cholesterol. Certain isolated H. pylori lipids (by themselves or when mixed with other lipids) also had the ability to form ordered domains. To be specific, H. pylori cholesteryl-6-O-tetradecanoyl-α-D-glucopyranoside (CAG) and cholesterol-6-O-phosphatidyl-α-D-glucopyranoside (CPG) had the ability to form and/or stabilize ordered domain formation, while H. pylori phosphatidylethanolamine did not, behaving similarly to unsaturated phosphatidylethanolamines. We conclude that specific H. pylori cholesterol lipids have a marked ability to form ordered lipid domains.<br /> (Copyright © 2019 Elsevier B.V. All rights reserved.)
- Subjects :
- Cell Membrane chemistry
Cell Membrane metabolism
Cholesterol chemistry
Fluorescence Resonance Energy Transfer methods
Lipid Metabolism physiology
Lipids chemistry
Membrane Lipids chemistry
Membrane Microdomains metabolism
Membrane Microdomains physiology
Membranes chemistry
Phosphatidylcholines analysis
Phosphatidylethanolamines analysis
Helicobacter pylori metabolism
Lipids physiology
Membrane Microdomains chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1879-2642
- Volume :
- 1861
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- Biochimica et biophysica acta. Biomembranes
- Publication Type :
- Academic Journal
- Accession number :
- 31449801
- Full Text :
- https://doi.org/10.1016/j.bbamem.2019.183050