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Identification of Differentially Expressed Proteins from Smokeless Tobacco Addicted Patients Suffering from Oral Squamous Cell Carcinoma.
Identification of Differentially Expressed Proteins from Smokeless Tobacco Addicted Patients Suffering from Oral Squamous Cell Carcinoma.
- Source :
-
Pathology oncology research : POR [Pathol Oncol Res] 2020 Jul; Vol. 26 (3), pp. 1489-1497. Date of Electronic Publication: 2019 Aug 24. - Publication Year :
- 2020
-
Abstract
- Oral squamous cell carcinoma (OSCC) is the eight most common malignancy worldwide with an incidence rate of 40% in south-east Asia. Lack of effective diagnostic tools at early stage and disease recurrence despite extensive treatments are main reasons for high mortality and low survival rates. The aim of current study was to identify differentially expressed proteins to explore potential candidate biomarkers having diagnostic significance. We performed comparative proteomic analysis of paired protein samples (cancerous buccal mucosa and adjacent normal tissue) from OSCC patients using a combination of two dimensional gel electrophoresis and Mass spectrometric analysis. On the basis of spot intensity, seventeen proteins were found to be consistently differentially expressed among most of the samples which were identified through mass spectrometry. For validation of identified proteins, expression level of stratifin was determined using immuno-histochemistry and Western blot analysis. All identified proteins were analyzed by STRING to explore their interaction. Among uniquely identified proteins in this study, at least two candidate markers (Ig Kappa chain C region and Isoform 2 of fructose bisphosphate aldolase A) were found to be novel with respect to OSCC which can be explored further. Results presented in current study are likely to contribute in understanding the involvement of these molecules in carcinogenesis apart from their plausible role as diagnostic/prognostic markers.
- Subjects :
- Adult
Aged
Biomarkers, Tumor metabolism
Female
Humans
Male
Middle Aged
Mouth Neoplasms etiology
Mouth Neoplasms pathology
Squamous Cell Carcinoma of Head and Neck etiology
Squamous Cell Carcinoma of Head and Neck pathology
Biomarkers, Tumor analysis
Mouth Neoplasms metabolism
Squamous Cell Carcinoma of Head and Neck metabolism
Tobacco, Smokeless adverse effects
Subjects
Details
- Language :
- English
- ISSN :
- 1532-2807
- Volume :
- 26
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Pathology oncology research : POR
- Publication Type :
- Academic Journal
- Accession number :
- 31446608
- Full Text :
- https://doi.org/10.1007/s12253-019-00724-y