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Non-morphogenic effect of Sonic Hedgehog on gastric H + ,K + -ATPase activity.
- Source :
-
Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2019 Oct 20; Vol. 518 (3), pp. 605-609. Date of Electronic Publication: 2019 Aug 21. - Publication Year :
- 2019
-
Abstract
- In the stomach, Sonic Hedgehog (Shh) is highly expressed in gastric parietal cells, and acts as a morphogen in early development of the organ. Here, we found that the cleaved N-terminal fragment of Shh (Shh-N) was abundantly expressed in hog gastric vesicles derived from the apical membrane of parietal cells. Interestingly, Shh-N recombinant significantly decreased K <superscript>+</superscript> -dependent ATP-hydrolyzing activity, which is sensitive to an inhibitor of H <superscript>+</superscript> ,K <superscript>+</superscript> -ATPase (SCH28080), in hog gastric tubulovesicles and membrane fractions of the H <superscript>+</superscript> ,K <superscript>+</superscript> -ATPase-expressing cells. In the living cells, Shh-N recombinant inhibited the SCH28080-sensitive <superscript>86</superscript> Rb <superscript>+</superscript> -uptake. Together, Shh-N may directly bind to extracellular side of H <superscript>+</superscript> ,K <superscript>+</superscript> -ATPase, and negatively regulates the pump activity. This is the first report to explore non-morphogenic property of Shh on ion transporters.<br /> (Copyright © 2019 Elsevier Inc. All rights reserved.)
Details
- Language :
- English
- ISSN :
- 1090-2104
- Volume :
- 518
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Biochemical and biophysical research communications
- Publication Type :
- Academic Journal
- Accession number :
- 31445709
- Full Text :
- https://doi.org/10.1016/j.bbrc.2019.08.099