Non-morphogenic effect of Sonic Hedgehog on gastric H + ,K + -ATPase activity.

Authors :: Fujii T
Phutthatiraphap S
Shimizu T
Takeshima H
Sakai H
Source :: Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2019 Oct 20; Vol. 518 (3), pp. 605-609. Date of Electronic Publication: 2019 Aug 21.
Publication Year :: 2019
Abstract: In the stomach, Sonic Hedgehog (Shh) is highly expressed in gastric parietal cells, and acts as a morphogen in early development of the organ. Here, we found that the cleaved N-terminal fragment of Shh (Shh-N) was abundantly expressed in hog gastric vesicles derived from the apical membrane of parietal cells. Interestingly, Shh-N recombinant significantly decreased K <superscript>+</superscript> -dependent ATP-hydrolyzing activity, which is sensitive to an inhibitor of H <superscript>+</superscript> ,K <superscript>+</superscript> -ATPase (SCH28080), in hog gastric tubulovesicles and membrane fractions of the H <superscript>+</superscript> ,K <superscript>+</superscript> -ATPase-expressing cells. In the living cells, Shh-N recombinant inhibited the SCH28080-sensitive <superscript>86</superscript> Rb <superscript>+</superscript> -uptake. Together, Shh-N may directly bind to extracellular side of H <superscript>+</superscript> ,K <superscript>+</superscript> -ATPase, and negatively regulates the pump activity. This is the first report to explore non-morphogenic property of Shh on ion transporters.<br /> (Copyright © 2019 Elsevier Inc. All rights reserved.)