Semi-Field Evaluation of Modified 00ZZZero ® Traps with Sticky Paper to Increase the Collection Efficacy of Gravid Aedes aegypti .

The 00ZZZero ^® trap (0Z) is a small black plastic container with permethrin and pyriproxyfen, designed and marketed to both attract and kill gravid Aedes mosquitoes (unmodified trap ([UMT] or modified trap [MT]). The 0Z trap without the insecticide was modified with addition of a sticky paper, coated with an adhesive glue (MT) and evaluated for collection efficacy of released gravid mosquito, Aedes aegypti (L.) in outdoor screened enclosures in St. Augustine, in northeastern Florida. The mean numbers (±SE) of mosquitoes caught by MTs were 130.67 ± 23.95 (40.22 ± 5.78 mosquito/trap), compared with 2.33 ± 0.88 (0.79 ± 0.29 mosquito/trap) mosquitoes caught by UMTs. The MTs collected significantly more mosquitoes, 44.16% ( F = 4.495, P < 0.05) of the released mosquitoes, than those captured in UMTs (0.83%). The number of immature mosquitoes (larvae and pupae) hatched from eggs laid in the MTs and UMTs were 79.3 ± 23.1 and 19.3 ± 6.8, respectively. The mean numbers (±SE) of adults emerging in MTs and UMTs were 34.0 ± 22.3 and 14.0 ± 6.8, respectively. These results show the potential of the sticky paper modified 0Z traps to be used both to increase the efficacy of collection and as a sticky autocidal tool against gravid Ae. aegypti .