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Proresolving Mediators LXB4 and RvE1 Regulate Inflammation in Stromal Cells from Patients with Shoulder Tendon Tears.
- Source :
-
The American journal of pathology [Am J Pathol] 2019 Nov; Vol. 189 (11), pp. 2258-2268. Date of Electronic Publication: 2019 Aug 19. - Publication Year :
- 2019
-
Abstract
- Tendon stromal cells isolated from patients with chronic shoulder rotator cuff tendon tears have dysregulated resolution responses. Current therapies do not address the biological processes concerned with persistent tendon inflammation; therefore, new therapeutic approaches that target tendon stromal cells are required. We examined whether two specialized proresolving mediators (SPMs), lipoxin B4 (LXB4) and resolvin E1 (RvE1), modulate the bioactive lipid mediator profiles of IL-1β-stimulated tendon cells derived from patients with shoulder tendon tears and healthy volunteers. We also examined whether LXB4 or RvE1 treatments moderated the proinflammatory phenotype of tendon tear stromal cells. Incubation of IL-1β-treated patient-derived tendon cells in LXB4 or RvE1 up-regulated concentrations of SPMs. RvE1 treatment of diseased tendon stromal cells increased 15-epi-LXB4 and regulated postaglandin F2α. LXB4 or RvE1 also induced expression of the SPM biosynthetic enzymes 12-lipoxygenase and 15-lipoxygenase. RvE1 treatment up-regulated the proresolving receptor human resolvin E1 compared with vehicle-treated cells. Incubation in LXB4 or RvE1 moderated the proinflammatory phenotype of patient-derived tendon tear cells, regulating markers of tendon inflammation, including podoplanin, CD90, phosphorylated signal transducer and activator of transcription 1, and IL-6. LXB4 and RvE1 counterregulate inflammatory processes in tendon stromal cells, supporting the role of these molecules as potential therapeutics to resolve tendon inflammation.<br /> (Copyright © 2019 American Society for Investigative Pathology. Published by Elsevier Inc. All rights reserved.)
- Subjects :
- Aged
Anti-Inflammatory Agents pharmacology
Cells, Cultured
Eicosapentaenoic Acid pharmacology
Female
Humans
Inflammation metabolism
Inflammation pathology
Inflammation prevention & control
Inflammation Mediators metabolism
Lacerations metabolism
Lacerations pathology
Male
Middle Aged
Shoulder pathology
Shoulder Injuries metabolism
Shoulder Joint drug effects
Shoulder Joint metabolism
Shoulder Joint pathology
Stromal Cells metabolism
Stromal Cells pathology
Tendon Injuries metabolism
Tendons metabolism
Tendons pathology
Eicosapentaenoic Acid analogs & derivatives
Lipoxins pharmacology
Shoulder Injuries pathology
Stromal Cells drug effects
Tendon Injuries pathology
Tendons drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 1525-2191
- Volume :
- 189
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- The American journal of pathology
- Publication Type :
- Academic Journal
- Accession number :
- 31437425
- Full Text :
- https://doi.org/10.1016/j.ajpath.2019.07.011